角果木内生真菌Cladosporium sp. JJM22次级代谢产物研究  

Study on secondary metabolites of endophytic fungus Cladosporium sp. JJM22 hosted in Ceriops tagal

作　　者：李臻 杨静雨 蔡瑾 欧阳紫娟 周楚涵 陈光英[1] 周学明[1] LI Zhen;YANG Jing-yu;CAJ Jin;OUYANG Zi-juan;ZHOU Chu-han;CHEN Guang-ying;ZHOU Xue-ming(Key Laboratory of Tropical Medicinal Resource Chemistry of Ministry of Education,Key Laboratory of Tropical Medicinal Plant Chemistry of Hainan Province,Hainan Normal University,Haikou 571158,China)

机构地区：[1]海南师范大学热带药用资源化学教育部重点实验室,海南省热带药用植物化学重点实验室,海南海口571158

出　　处：《中国中药杂志》2021年第8期2079-2083,共5页China Journal of Chinese Materia Medica

基　　金：海南省自然科学基金青年项目(218QN234);教育部创新团队项目(IRT-16R1)。

摘　　要：综合运用多种柱色谱在活性追踪的指导下对角果木内生真菌Cladosporium sp. JJM22次级代谢产物进行分离纯化,并根据化合物的理化性质以及NMR,MS数据进行结构鉴定,共分离鉴定了9个化合物,(S)-5-hydroxy-4-methylchroman-2-one (1),4-methoxynaphthalene-1,5-diol (2),8-methoxynaphthalene-1,7-diol (3),1,8-dimethoxynaphthalene (4),(2R,4S)-2,3-dihydro-2-methyl-benzopyran-4,5-diol (5),(2R,4R)-3,4-dihydro-4-methoxy-2-methyl-2H-1-benzopyran-5-ol (6), 7-O-α-D-ribosyl-2,3-dihydro-5-hydroxy-2-methyl-chromen-4-one (7),(R)-3-methoxyl-1-(2,6-dihydroxyphenyl)-butan-1-one (8)和helicascolide A (9)。化合物1为新化合物,化合物2为新天然产物。抗炎活性表明化合物2~4对LPS诱导RAW264.7细胞NO生成具有较好的抑制作用。Nine secondary metabolites(S)-5-hydroxy-4-methylchroman-2-one(1), 4-methoxynaphthalene-1,5-diol(2), 8-methoxynaphthalene-1,7-diol(3), 1,8-dimethoxynaphthalene(4),(2R,4S)-2,3-dihydro-2-methyl-benzopyran-4,5-diol(5),(2R,4R)-3,4-dihydro-4-methoxy-2-methyl-2H-1-benzopyran-5-ol(6), 7-O-α-D-ribosyl-2,3-dihydro-5-hydroxy-2-methyl-chromen-4-one(7),(R)-3-methoxyl-1-(2,6-dihydroxyphenyl)-butan-1-one(8) and helicascolide A(9) were isolated from endophytic fungus Cladosporium sp. JJM22 by using column chromatographies of silica gel and ODS, and semi-preparative HPLC. Their structures were analyzed on the basis of spectroscopic and chemical data, especially NMR and MS. All isolated compounds were evaluated for their anti-inflammatory activities by examining the inhibitory activities on nitric oxide(NO) production induced by lipopolysaccharide in mouse macrophage RAW264.7 cells in vitro. Compounds 2-4 showed inhibitory activities.

关 键 词：内生真菌 CLADOSPORIUM 次级代谢产物 抑制NO生成 

分 类 号：R284[医药卫生—中药学]