运动通过p38MAPK信号通路影响成长期小鼠骨代谢  

Exercise effects bone metabolism of growing mice through p38MAPK signaling pathway

作　　者：凌丽丽 李桂芳 王彪 赵学辉 刘会领 李建英 王新梅 LING Li-li;LI Gui-fang;WANG Biao;ZHAO Xue-hui;LIU Hui-ling;LI Jian-ying;WANG Xin-mei(Department of Neonatal,Cangzhou People's Hospital;Interpol Detachment,Cangzhou Public Security Bureau of Hebei Province,Cangzhou 061000,Hebei,China)

机构地区：[1]沧州市人民医院新生儿科 [2]沧州市公安局刑警支队,河北沧州061000

出　　处：《川北医学院学报》2021年第5期557-561,共5页Journal of North Sichuan Medical College

基　　金：河北省沧州市科学技术局(151302067)。

摘　　要：目的:分析运动如何通过p38丝裂原活化蛋白激酶(p38MAPK)信号通路影响成长期小鼠骨代谢。方法:40只8周龄C57/BL6雄性小鼠随机分为运动组和对照组,每组各20只。运动组小鼠在跑台上进行8周下坡跑;检测两组小鼠血清生化指标、右侧股骨生物力学指标、血小板衍生生长因子B(PDGFb)、c-fos蛋白、细胞外调节蛋白激酶-1(ERK-1)、骨成型蛋白2(BMP-2)、磷酸化丝裂原活化蛋白激酶(P-MAPK)、黑质区磷酸化p38(p-P38)、黑质环氧合酶-2(COX-2)、半胱氨酸蛋白酶-3(caspase-3)的表达水平。结果:运动组小鼠的血清钙(Ca)、碱性磷酸酶(AKP)、抗酒石酸酸性磷酸酶(STR-ACP)、骨钙素(BGP)、碱性磷酸酶(ALP)的水平均高于对照组(P<0.05),而血清磷(P)水平低于对照组(P<0.05);运动组小鼠的各股骨生物力学指标均优于对照组(P<0.05);运动组小鼠的PDGFb、c-fos、ERK-1、BMP-2、P-MAPK蛋白的水平均高于对照组(P<0.05);运动组小鼠的黑质区p-P38、COX-2、caspase-3水平均高于对照组,差异具有统计学意义(P<0.05)。结论:运动能够激活成长期小鼠的p38MAPK信号通路相关细胞因子表达,改善骨代谢,增加骨密度。Objective:To analyze how exercise effects bone metabolism of growing mice through p38 mitogen activated protein kinase(p38MAPK)signaling pathway.Methods:40 C57/BL6 male rats(8 weeks old)were selected and divided into two groups by random number table method,namely exercise group and control group,20 cases in each group,and the exercise group mice ran downhill on the treadmill for 8 weeks.The serum biochemical indexes,right femoral biomechanical indexes,platelet-derived growth factor B(PDGFb),c-fos protein,extracellular regulatory protein of the exercise group mice Kinase-1(ERK-1),bone morphogenetic protein-2(BMP-2),phosphorylated mitogen-activated protein kinase(P-MAPK),phosphorylated p38(p-P38),cyclooxygenase-2(COX-2)and cysteine protease-3(caspase-3)in substantia nigra were detected.Results:The levels of serum calcium(Ca),alkaline phosphatase(AKP),tartrate-resistant acid phosphatase(STR-ACP),osteocalcin(BGP)and alkaline phosphatase(ALP)in the exercise group were higher than those in the control group(P<0.05).The serum phosphorus(P)level was lower than the control group(P<0.05),the femoral biomechanical indexes of the exercise group mice were better than that of the control group(P<0.05).The PDGFb,c-fos,ERK-1,BMP-2 and P-MAPK protein expression levels in the exercise group were higher than those in the control group(P<0.05).The levels of p-P38,COX-2 and caspase-3 in the exercise group were higher than those in the control group,and the differences were statistically significant(P<0.05).Conclusion:Sports can activate the expression of p38MAPK signaling pathway related cytokines,improve bone metabolism and increase bone mineral density in growing mice.

关 键 词：骨代谢 成长期 运动 P38MAPK信号通路 

分 类 号：G804.2[文化科学—运动人体科学] G804.7[文化科学—体育学]