番茄MYB44基因生物信息学及亚细胞定位分析  

MYB44 Gene from Solanum Lycopersicum:Bioinformatics Analysis and Subcellular Localization

作　　者：李颖 王莹莹 苏旭 谷建田 Li Ying;Wang Yingying;Su Xu;Gu Jiantian(College of Plant Science and Technology,Beijing Agricultural University,Beijing 102206)

机构地区：[1]北京农学院植物科学技术学院,北京102206

出　　处：《中国农学通报》2021年第14期34-40,共7页Chinese Agricultural Science Bulletin

基　　金：北京市教委科技发展项目“番茄辐射突变体低温耐受性的转录组分析及差异基因表达模式研究”(5075227129/015)。

摘　　要：旨在分析SlMYB44基因的生物学功能,为其抗冷害作用机制提供科学依据。本文以俄罗斯栽培番茄Bolgogragsky为材料,根据转录组测序结果,筛选出差异表达基因SlMYB44作为研究对象。运用多种生物信息学数据库分析了番茄SlMYB44基因的保守结构域、亲疏水性、信号肽以及启动子顺式作用元件。并且构建了pMDC43-MYB44表达载体进行亚细胞定位分析。结果表明SlMYB44基因全长1551 bp,共372个氨基酸。SlMYB44蛋白没有潜在的信号肽和跨膜结构,属于不稳定、亲水性蛋白。SlMYB44基因启动子顺式作用元件分析发现,大部分与逆境胁迫、激素响应等相关。亚细胞定位分析发现SlMYB44基因表达产物定位于细胞核中。SlMYB44基因对番茄冷害有响应,进一步推测其可能影响番茄御冷机制。The aims are to analyze the biological function of Sl MYB44 gene and provide a scientific basis for exploring its mechanism of resisting chilling injury.In this study,the Russian cultivated tomato bolgogragsky was used as the material,and according to the sequencing results of transcriptome,the differentially expressed gene Sl MYB44 was selected as the research object.The conservative functional domain,hydrophilicity or hydrophobicity,signal peptide and promoter cis-acting elements of tomato Sl MYB44 gene were analyzed by using various bioinformatics databases.The expression vector p MDC43-MYB44 was constructed for subcellular localization analysis.Sl MYB44 gene encoded 372 amino acids with a total length of 1551 bp.Sl MYB44 protein had no potential signal peptide site and membrane spaning domain,which belonged to labile protein and hydrophilic protein.The analysis of cis-acting elements of Sl MYB44 gene promoter showed that most of them were related to adversity stress and hormone response.Subcellular localization analysis demonstrated that the expression product of Sl MYB44 gene was localized in the cell nucleus.In conclusion,the Sl MYB44 gene responds to chilling injury,it is further speculated that it might affect the tomato chilling mechanism.

关 键 词：番茄 基因 SlMYB44 生物信息学分析 亚细胞定位 低温胁迫 

分 类 号：S641.2[农业科学—蔬菜学]