Vanillin and its derivatives,potential promising antifungal agents,inhibit Aspergillus flavus spores via destroying the integrity of cell membrane rather than cell wall  

作　　者：Qian Li Xiaoman Zhu 

机构地区：[1]Henan Key Laboratory of Cereal and Oil Food Safety Inspection and Control,College of Food Science and Engineering,Henan University of Technology

出　　处：《Grain & Oil Science and Technology》2021年第2期54-61,共8页粮油科技（英文版）

基　　金：the financial support of the Doctor Research Fund of Henan University of Technology(2019BS019);the Natural Science Research Projects of Education Department of Henan Province(21A550005);the Innovative Funds plan of Henan University of Technology(2020ZKCJ17)。

摘　　要：To study the structure-antifungal activity of vanillin against Aspergillus flavus(A.flavus),the susceptibilities of A.flavus to vanillin and its isomers(o-vanillin,2-hydroxy-4-methoxybenzaldehyde(HMB),2-hydroxy-5-methoxybenzaldehyde)and the possible antifungal mechanisms have been investigated.All the four volatile aldehydes could inhibit the germination of spores,and the minimum inhibitory concentrations(MICs)of them were in this order:vanillin(200μg/mL),o-vanillin(100μg/mL),2-hydroxy-5-methoxybenzaldehyde(100μg/mL),HMB(70μg/mL).The minimum fungicidal concentrations(MFCs)of them were in this order:vanillin(240μg/mL),o-vanillin(160μg/mL),HMB(140μg/mL),2-hydroxy-5-methoxybenzaldehyde(140μg/mL).Spore size was arrested at 0 h with the treatment of the four volatile aldehydes.Effects of the four volatile aldehydes on the cell wall and cell membrane integrity of A flavus were observed by calcofluor white(CW)staining and propidium iodide(PI)staining.The results showed that HMB exerted the strongest antifungal and fungicidal effects on the growth of A.flavus.The four volatile aldehydes had little influence on cell wall integrity after 3-hour treatment,however,they could strongly damage the cell membrane integrity.All the four volatile aldehydes could effectively prevent the growth of A.flavus on peanut seeds.The antifungal mechanisms of the four volatile aldehydes provide theoretical foundations for their development of new antifungal agents.

关 键 词：Aspergillus flavus VANILLIN O-VANILLIN HMB 2-Hydroxy-5-methoxybenzaldehyde Antifungal mechanism 

分 类 号：TQ450.1[化学工程—农药化工]