烟草工业废水和烟草浓缩液中甾体雌激素的生物降解研究  

Study on biodegradation of steroidal estrogen in tobacco industrial wastewater and tobacco concentrate

作　　者：孙福艳 叶建斌 马歌丽 齐晓娜 张展 冯颖杰 杨峰 SUN Fuyan;YE Jianbin;MA Geli;QI Xiaona;ZHANG Zhan;FENG Yingjie;YANG Feng(College of Food and Bioengineering,Zhengzhou University of Light Industry,Zhengzhou 450001,China;China Tobacco He′nan Industrial Co.,Ltd.,Zhengzhou 450002,China;He′nan Cigarette Industry Tobacco Sheet Co.,Ltd.,Xuchang 461000,China)

机构地区：[1]郑州轻工业大学食品与生物工程学院,河南郑州450001 [2]河南中烟工业有限责任公司,河南郑州450002 [3]河南卷烟工业烟草薄片有限责任公司,河南许昌461000

出　　处：《轻工学报》2021年第3期54-62,共9页Journal of Light Industry

基　　金：国家自然科学基金项目(4180071401)。

摘　　要：从烟草工业废水处理厂的污泥中筛选出一株以雌酮(E1)为底物的甾体雌激素降解菌株ye22,16S rRNA鉴定该菌株为琼氏不动杆菌,对其生长特性及降解动力学进行研究.结果表明,该菌株在以E1为唯一碳源的培养基中,发酵9 d对E1的降解率达41.88%;烟草工业废水和烟草浓缩液中E1质量浓度较高,分别达到1.41×10^(-4) g/L和9.01×10^(-5) g/L;收集培养到对数期的ye22菌株,接种于烟草工业废水和烟草浓缩液中,发酵9 d,E1降解率分别为32.07%和27.23%.A steroid-estrogen-degrading strain ye22 with Estone(E1)as substrate was screened out from tobacco industry wastewater treatment plant sludge.It was identified as Acinetobacter junii by 16S rRNA sequencing.The results showed that the growth characteristics and degradation kinetics of the strain were studied.After 9 days fermentation,the degradation rate of E1 could reach 41.88%.The concentration of E1 in tobaccoindustrial wastewater and tobacco concentrate was higher,reaching 1.41×10^(-4) g/L and 9.01×10^(-5) g/L,respectively.The ye22 strain cultured to logarithmic phrase could degrade 32.07%of E1 in tobacco industry wastewater and 27.23%of E1 in tobacco concentrate within 9 days.

关 键 词：烟草 甾体雌激素 生物降解 废水处理 

分 类 号：TS48[农业科学—烟草工业] X795[环境科学与工程—环境工程]