马铃薯PLRV病毒宁夏分离物外壳蛋白基因CP的克隆与序列分析  

Molecular Biological Identification of Potato Leafroll Virus from Field Samples in Ningxia Province

作　　者：陈晓军 郭成瑾 王喜刚 沈瑞清 CHEN Xiao-jun;GUO Cheng-jin;WANG Xi-gang(Agricultural Biotechnology Center,Ningxia Academy of Agriculture and Forestry Sciences/Key Lab of Agricultural Biotechnology of Ningxia,Yinchuan,Ningxia 750002;Institute of Plant Protection,Ningxia Academy of Agriculture and Forestry Sciences/Key Laboratory of Plant Disease and Pest Control,Yinchuan,Ningxia 750002)

机构地区：[1]宁夏农林科学院农业生物技术研究中心/宁夏农业生物技术重点实验室,宁夏银川750002 [2]宁夏农林科学院植物保护研究所/宁夏植物病虫害防治重点实验室,宁夏银川750002

出　　处：《安徽农业科学》2021年第11期96-99,共4页Journal of Anhui Agricultural Sciences

基　　金：宁夏自然基金项目“SYBR Green I实时荧光定量PCR检测马铃薯炭疽病菌体系的建立与应用”(2020AAC03316);宁夏农林科学院科技创新引导项目“高效作物土传病害微生物生态调控剂研制”(NKYG-18-07)。

摘　　要：利用双抗体夹心酶联免疫吸附法分离得到了PLRV宁夏分离物,并采用RT-PCR技术分离得到了宁夏分离物外壳蛋白基因CP的核苷酸序列,PLRV CP基因共624 bp,推测编码208个氨基酸残基,分子量大小为23234.2,等电点11.85。通过同源性分析,其与2013年内蒙古分离物(KC456052)最为接近,同源性达99.8%;进化树分析发现其有一定的地理区域性,为单独分支起源。PLRV CP gene was isolated from Ningxia by DAS-ELISA method.The nucleotide sequence of CP gene of PLRV isolated from Ningxia was obtained by RT-PCR.The total length of PLRV CP gene was 624 bp.It was inferred that PLRV CP gene encoded 208 amino acid residues with molecular weight of 23234.2 and pI of 11.85.Through homology analysis,it was the closest to the isolate(KC456052)from Inner Mongolia in 2013,with 99.8%homology;phylogenetic tree analysis showed that it had a certain geographical region,which was a single branch origin.

关 键 词：马铃薯卷叶病病毒 反转录 宁夏PLRV分离物 

分 类 号：S435.32[农业科学—农业昆虫与害虫防治] S432.4[农业科学—植物保护]