景宁木兰PIF转录因子的生物信息学分析及极端遮阴条件下的表达模式  

Bioinformatics analysis of PIF transcription factors in Magnolia sinostellata and expression pattern analysis under extreme shading condition

作　　者：陆丹迎 程少禹 章颖佳 刘志高[1] 金梦婷 董彬[1] 张寿洲[2] 彭豪 戴梦怡 王卓为 赵宏波[1] 申亚梅[1] LU Danying;CHENG Shaoyu;ZHANG Yingjia;LIU Zhigao;JIN Mengting;DONG Bin;ZHANG Shouzhou;PENG Hao;DAI Mengyi;WANG Zhuowei;ZHAO Hongbo;SHEN Yamei(College of Landscape and Architecture,Zhejiang A&F University,Hangzhou 311300,Zhejiang,China;Fairylake Botanical Garden,Shenzhen&Chinese Academy of Sciences,Shenzhen 518004,Guangdong,China)

机构地区：[1]浙江农林大学风景园林与建筑学院,浙江杭州311300 [2]深圳市/中国科学院仙湖植物园,广东深圳518004

出　　处：《浙江农林大学学报》2021年第3期445-454,共10页Journal of Zhejiang A&F University

基　　金：“十三五”浙江省林木新品种选育专项(2016C02056-1);浙江省重点研发项目(2019C02023)。

摘　　要：【目的】群落所造成的遮阴是导致景宁木兰Magnolia sinostellata濒危的重要因素之一。PIF家族转录因子在光信号传导和植物生长发育中起到重要作用。对PIF家族转录因子进行系统分析和研究,为探究其在景宁木兰光信号转导机制中的作用奠定基础。【方法】从景宁木兰转录组数据中鉴定获得PIF家族转录因子并进行生物信息学分析,利用实时荧光定量聚合酶链式反应(qRT-PCR)技术对其在极端遮阴条件下的表达模式进行分析。【结果】从景宁木兰转录组中共筛选出9个MsPIFs转录因子基因,其编码的蛋白质长度为188~735个氨基酸,蛋白质大小为20 314.56~78 957.02 Da,理论等电点范围为5.18~8.22。MsPIFs基因编码的蛋白质均为不稳定蛋白质,所有蛋白质均为亲水性蛋白质。亚细胞定位预测结果显示所有蛋白质均定位于细胞核。9个蛋白质均具有丝氨酸(Ser)、苏氨酸(Thr)和酪氨酸(Try)磷酸化位点。qRT-PCR结果表明:极端遮阴条件下,9个MsPIFs家族基因表达均发生不同程度的变化。其中,MsbHLH23的表达变化较其他基因更为明显,遮阴处理5和10 d时的表达量分别上调为对照的52.77与20.03倍。【结论】景宁木兰PIF转录因子家族均能响应遮阴,为后续对MsPIFs进行生物学功能鉴定奠定了基础。[Objective] The shading caused by the community is one of the important factors that lead to the endangerment of Magnolia sinostellata. Therefore, it is important to conduct a systematic analysis and research of PIF family transcription factors which play an important role in light signal transduction and plant growth.Also, such analysis will help lay a foundation for the exploration of its role in the light signal transduction mechanism of M. sinostellata. [Method] With the transcriptome data of M. sinostellata collected, transcription factors of PIF family were identified and analyzed by bioinformatic while the expression patterns were analyzed employing the qRT-PCR technology. [Result] The nine MsPIFs transcription factor genes screened from theM. sinostellata enjoyed a length of 188-735 aa, a protein size of 20 314.56-78 957.02 Da, and a theoretical isoelectric point range of 5.18-8.22. The proteins encoded by Ms PIFs gene were unstable proteins, and all proteins were hydrophilic proteins localized in the nucleus as was demonstrated in the subcellular localization prediction. All nine proteins have Ser, Thr and Try phosphorylation sites. As was shown in the q RT-PCR results, under extreme shading conditions, there was the occurrence of changes of different degrees in the gene expression of 9 Ms PIFs families of which MSBHLH23 has an expression level that was 52.77 and 20.03 times higher than that of the control at 5 d and 10 d after shading treatment. [Conclusion] The PIF transcription factor family of M. sinostellata can respond to shading and this study has laid a foundation for the identification of Ms PIFs biological function.

关 键 词：景宁木兰 极端遮阴 PIF转录因子家族 生物信息学分析 表达模式 

分 类 号：S718.3[农业科学—林学]