中医药调节肠道微生态研究技术进展  

Advances in research technology of regulation of intestinal microecology by traditional Chinese medicine

作　　者：李小雅 谭周进[1] Xiao-Ya Li;Zhou-Jin Tan(Hunan University of Chinese Medicine,Changsha 410208,Hunan Province,China)

机构地区：[1]湖南中医药大学,湖南省长沙市410208

出　　处：《世界华人消化杂志》2021年第9期479-487,共9页World Chinese Journal of Digestology

基　　金：国家自然科学基金项目,No.81874460.

摘　　要：肠道是一个多元化的微生态系统,其结构及功能是生命科学及医学领域的研究热点,中医药调节肠道微生态的研究对中医基础理论、中医药疗效机制等的阐明具有重要意义.肠道微生态研究技术对该领域研究至关重要,笔者通过查阅相关资料,对中医药调节肠道微生态研究的相关技术如微生物培养技术、分子生物学技术(变性/温度梯度凝胶电泳技术(DGGE/TGGE)、基因芯片技术(Gene chip)、实时荧光定量PCR技术(PT-PCR)、末端限制性片段长度的多态性技术(T-RFLP)、扩增核糖体DNA限制性酶切片段分析技术(ARDRA)、荧光原位杂交技术(FISH)、高通量测序技术(HTS)、代谢水平分析技术、蛋白质组学技术)、肠道酶活性检测、肠道微生物活度检测技术以及体外模拟胃肠道环境实验进行总结,对中医药肠道微生态的研究及相关技术的应用提供参考.The intestine is a diversified microecosystem whose structure and function are research hotspots in the fields of life sciences and medicine.The research on the regulation of intestinal microecology by traditional Chinese medicine is of great significance to the elucidation of the basic theories and the mechanism of therapeutic effects of traditional Chinese medicine.The micro-ecological research technology is very important for the research in this field.We,by reviewing the relevant literature,summarize the related technologies for the intestinal micro-ecological research of traditional Chinese medicine,such as microbial culture counting method,molecular biology techniques(denaturing/temperature gradient gel electrophoresis(DGGE/TGGE),gene chip,real-time quantitative polymerase chain reaction(PT-PCR),terminal-restriction fragment length polymorphism(T-RFLP),amplified ribosomal DNA restriction analysis(ARDRA),fluorescent in situ hybridization(FISH),high-throughput sequencing(HTS),metabolic level analysis techniques,and proteomics),intestinal enzyme activity detection,microbial activity detection technology,and in vitro simulated gastrointestinal environment experiments,with an aim to provide reference for the intestinal micro-ecological research of traditional Chinese medicine and the application of related technologies.

关 键 词：肠道微生态 中医药 研究技术 微生物培养计数法 分子生物学技术 

分 类 号：R259[医药卫生—中西医结合]