Tetramethylpyrazine inhibits proliferation of colon cancer cells in vitro  

作　　者：Hua Li Yan-Xu Hou Yu Yang Qing-Qiang He Tian-Hua Gao Xiao-Feng Zhao Zhi-Bin Huo Shu-Bo Chen Deng-Xiang Liu 

机构地区：[1]Institute of Cancer Control,Xingtai People’s Hospital,Xingtai 054001,Hebei Province,China [2]School of First Clinical Medicine,Nanjing University of Chinese Medicine,Nanjing 210029,Jiangsu Province,China

出　　处：《World Journal of Clinical Cases》2021年第18期4542-4552,共11页世界临床病例杂志

摘　　要：BACKGROUND Colon cancer is one of the most common malignancies worldwide,and chemotherapy is a widely used strategy in colon cancer clinical therapy.However,chemotherapy resistance is a major cause of disease recurrence and progression in colon cancer,and thus novel drugs for treatment are urgently needed.Tetramethylpyrazine(TMP),a component of the traditional Chinese medicine Chuanxiong Hort,has been proven to exhibit a beneficial effect in tumors.AIM To investigate the potential anticancer activity of TMP in colon cancer and its underlying mechanisms.METHODS Colon cancer cells were incubated with different concentrations of TMP.Cell viability was evaluated by crystal violet staining assay and cell counting kit-8 assay,and cell apoptosis and cell cycle were assessed by flow cytometry.RESULTS TMP significantly inhibited the proliferation of colon cancer cells in a dose-and time-dependent manner.In addition,flow cytometry revealed that TMP induced cell cycle arrest at the G0/G1 phase.TMP treatment caused early stage apoptosis in SW480 cells,whereas it caused late stage apoptosis in HCT116 cells.CONCLUSION Our studies demonstrated that TMP inhibits the proliferation of colon cancer cells in a dose-and time-dependent manner by inducing apoptosis and arresting the cell cycle at the G0/G1 phase.Our findings suggest that TMP might serve as a potential novel therapeutic drug in the treatment of human colon cancer.

关 键 词：TETRAMETHYLPYRAZINE Colon cancer Apoptosis Cell proliferation CHEMOTHERAPY Cell cycle 

分 类 号：R285[医药卫生—中药学]