矢志方对高尿酸血症大鼠尿酸和脂质代谢水平的影响  

Effect of Shizhi Prescription on Serum Uric Acid and Lipid Metabolism Level in Rats of Hyperuricemia

作　　者：吴燕升 谢芸[2] 周剑国[3] 刘秋玉 刘伟伟 高建东[1] WU Yansheng;XIE Yun;ZHOU Jianguo;LIU Qiuyu;LIU Weiwei;GAO Jiandong(Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Nephropathy Institute of Chinese Medicine,Shanghai University of Traditional Chinese Medicine,Key Laboratory of Liver and Kidney Diseases of Traditional Chinese Clinical Medicine,Shanghai 201203,China;Shanghai Ninth People’s Hospital,Shanghai Jiaotong University School of Medicine,Shanghai 200011,China;Sijing Hospital of Songjiang District,Shanghai 201601,China)

机构地区：[1]上海中医药大学附属曙光医院,上海中医药大学中医肾病研究所,上海市中医临床肝肾疾病重点实验室,上海201203 [2]上海交通大学医学院附属第九人民医院,上海200011 [3]上海市松江区泗泾医院,上海201601

出　　处：《中国中医药信息杂志》2021年第6期52-58,共7页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金(81904126、81874437、81373613)。

摘　　要：目的观察矢志方对不同病程高尿酸血症大鼠尿酸和脂质代谢水平的影响。方法 160只SD大鼠随机分为正常组、模型组、矢志方组和别嘌醇组,每组40只,除正常组外,其余各组给予氧嗪酸钾(750 mg/kg)灌胃制备高尿酸血症大鼠模型,矢志方组和别嘌醇组同时给予相应药物灌胃,实验第3、5、7、11周各组取10只大鼠检测肾功能(血尿酸、血尿素氮、血肌酐)、血脂[总胆固醇(TC)、三酰甘油(TG)、低密度脂蛋白胆固醇(LDL-C)]、血清及肝肾组织黄嘌呤氧化酶(XOD)活性及肾脏病理变化。结果与同一时间点正常组比较,模型组第11周TC、TG水平明显升高(P<0.05),各时间点LDL-C、血尿酸水平及XOD活性均显著升高(P<0.05),24 h尿酸排泄总量减少;与同一时间点模型组比较,矢志方组和别嘌醇组除第5周外血尿酸水平明显降低,血清及肝肾组织各时间点XOD活性明显降低(P<0.05,P<0.0001),矢志方组第3、11周24h尿酸排泄总量显著增加(P<0.05),矢志方组第11周TC、TG和LDL-C水平明显降低,各组大鼠血尿素氮及血肌酐水平差异均无统计学意义(P>0.05)。HE、PAS、Masson病理染色及电镜超微结构可见,矢志方可缓解肾小管炎症和纤维化改变。结论矢志方可降低不同病程高尿酸血症大鼠血尿酸水平,增加尿酸排泄量,改善脂质代谢,延缓肾小管间质纤维化。Objective To observe the effects of Shizhi Prescription on serum uric acid and lipid metabolism level in rats with different courses of hyperuricemia. Methods Totally 160 SD rats were divided into normal group, model group, Shizhi Prescription group and allopurinol group, with 40 rats in each group. Except for the normal group, the other groups were given potassium oxazine(750 mg/kg) per day to prepare hyperuricemia rat models. Shizhi Prescription group and the allopurinol group were given corresponding drugs simultaneously. On the 3 rd, 5 th, 7 th, and 11 th week of the experiment, 10 rats from each group were taken to test renal function(blood uric acid, blood urea nitrogen, blood creatinine), blood lipids(TC, TG, LDL-C), xanthine oxidation enzyme(XOD) activity of surem and kidney tissue and renal pathological changes. Results Compared with the normal group at the same time point, the TC and TG levels of the model group increased significantly at the 11 th week(P<0.05), and LDL-C levels increased significantly at each time point(P<0.05), the blood uric acid level and XOD activity were significantly increased(P<0.05), and the total amount of uric acid excretion in 24 h decreased. Compared with the model group at the same time point, the blood uric acid level of Shizhi Prescription group and allopurinol group was significantly reduced except the 5 th week, and the XOD activity of serum and liver and kidney tissue was significantly decreased at each time point(P<0.05, P<0.000 1). The total amount of uric acid excretion in the Shizhi Prescription group was significantly increased at the 3 rd and 11 th week(P<0.05). Shizhi Prescription group at 11 th week TC, TG, LDL-C was significantly decreased(P<0.05). There was no significant difference in the levels of blood urea nitrogen and blood creatinine in each group of rats(P>0.05). The pathological staining of HE, PAS, Masson and the ultrastructure of electron microscopy could be seen. Shizhi Prescription could alleviate renal tubular inflammation and fibrosis. Conclusi

关 键 词：高尿酸血症 矢志方 尿酸 脂质代谢 大鼠 

分 类 号：R285.5[医药卫生—中药学]