软骨特异性敲除SIRT1基因对小鼠椎体骨质疏松的影响及机制分析  

Effect of cartilage specific SIRT1 knockout on vertebral osteoporosis in mice and its mechanism analysis

作　　者：唐家国 覃松 王凯 邹凯 何精选 李晨芳 于滨生[3,4] Tang Jiaguo;Qin Song;Wang Kai;Zou Kai;He Jingxuan;Li Chenfang;Yu Binsheng(Department of Orthopedic Surgery,General Hospital of Yangze River Shipping,Wuhan Brain Hospital,Wuhan Hubei,430010;Department of Geriatrics,General Hospital of Yangze River Shipping,Wuhan Brain Hospital,Wuhan Hubei,430010;Shenzhen Key Laboratory of Spine Surgery,Department of Spine Surgery,Shenzhen Hospital,Peking University,Shenzhen Guangdong,518036;Shenzhen Engineering Laboratory of Orthopedic Regenerative Technologies,Orthopedics Research Center,Shenzhen Hospital,Peking University,Shenzhen Guangdong,518036,China)

机构地区：[1]长江航运总医院·武汉市脑科医院骨外科,湖北武430010 [2]长江航运总医院·武汉市脑科医院老年科,湖北武汉430010 [3]北京大学深圳医院脊柱外科·深圳市脊柱外科重点实验室,广东深圳518036 [4]北京大学深圳医院骨科研究中心·深圳市骨科疾病再生技术工程实验室,广东深圳518036

出　　处：《生物骨科材料与临床研究》2021年第3期30-35,共6页Orthopaedic Biomechanics Materials and Clinical Study

基　　金：湖北省卫生健康科研基金资助项目(WJ2019H388);武汉市卫生健康科研基金资助项目(WX19Q41)。

摘　　要：目的探讨沉默信息调节因子(SIRT1)基因对小鼠椎体骨质疏松的影响。方法将小鼠分为两组:软骨SIRT1基因未敲除小鼠组(A组,n=7);软骨SIRT1基因特异性敲除小鼠组(B组,n=7)。利用荧光定量聚合酶链反应及SIRT1免疫组化检测SIRT1基因的表达情况:通过对两组小鼠尾椎行Micro-CT扫描量化骨量体积百分比、骨小梁数量和骨小梁的厚度的改变;再采用HE染色、Masson染色评价椎体骨质结构的改变。结果B组小鼠SIRT1 mRNA表达量为(2.61±1.11),明显低于A组(8.08±1.64)(P<0.01);B组SIRT1蛋白的免疫组化染色积分为(3.10±2.69),明显低于A组(5.07±2.99)(P<0.01)。Micro-CT结果显示,A组小鼠骨量体积百分比为(37.96±8.54)%,显著高于B组的(28.39±6.27)%,差异有统计学意义(P<0.05);A组小鼠骨小梁数目(5.76±0.63)显著高于B组(5.15±0.50),差异有统计学意义(P<0.05);两组小鼠的骨小梁厚度差异无统计学意义(P>0.05)。HE染色和Masson染色结果显示,A组椎体骨质较B组明显密集。结论SIRT1基因敲除减少了小鼠椎体骨量体积百分比、骨小梁数量,因此SIRT1基因可能对小鼠椎体骨量调节具有积极的作用。Objective To investigate the effect of SIRT1 knock-out on vertebral osteoporosis in mice.Methods The mice were divided into two groups:Wild type control group(group A,n=7);Cartilage SIRT1 knockout group(group B,n=7).FQ-PCR and SIRT1 immunohistochemistry were used to detect the expression of SIRT1 gene;Micro-CT scan was performed on the tail vertebrae of the two groups to quantify percentage of bone mass,number of trabecular bone and thickness of trabecular bone.HE staining andMasson staining were used to evaluate the changes of vertebral bone structure.Results The expression of SIRT1 mRNA in group B was(2.61±1.11),which was significantly lower than that of group A(8.08±1.64)(P<0.01).The immunohistochemical staining score of SIRT1 protein in group B was(3.10±2.69),which was significantly lower than that of group A(5.07±2.99)(P<0.01).Micro-CT results showed that the bone volume percentage ofmice in group A[(37.96±8.54)%]was significantly higher than that in group B[(28.39±6.27)%],which was statistically significant(P<0.05);the number of trabecular bones in group A(5.76±0.63)was significantly higher than that of group B(5.15±0.50),which was statistically significant(P<0.05).There was no significant difference in trabecular thickness between two groups(P>0.05).The results of HE staining and Masson staining showed that the vertebral bone in group A was significantly denser than that in group B.Conclusion SIRT1 knockout reduced the volume percentage of vertebral body and the number of trabeculae in mice,so SIRT1 gene may have a positive regulatory effect on the bone mass of vertebral body in mice.

关 键 词：沉默信息调节因子1(SIRT1) 基因敲除 骨质疏松 动物实验研究 

分 类 号：R589[医药卫生—内分泌]