不同氧浓度下甲烷氧化耦合反硝化及微生物协作机制研究  

Methane oxidation coupled with denitrification and microbial cooperation mechanism under different oxygen concentrations

作　　者：赖生平 杨洪伟 徐志磊 聂明建 LAI Shengping;YANG Hongwei;XU Zhilei;NIE Mingjian(Chongqing Derun Environment Co.,Ltd.,Chongqing 400000,China;Central and Southem China Municipal Engineering Design&Research Institute Co.,Ltd.,Wuhan 430000,China)

机构地区：[1]重庆德润环境有限公司,重庆400000 [2]中国市政工程中南设计研究总院有限公司,湖北武汉430000

出　　处：《能源环境保护》2021年第3期46-52,共7页Energy Environmental Protection

摘　　要：为了研究不同氧浓度条件下甲烷氧化与反硝化的耦合关系,采用三套浸出床生物反应器模拟了准好氧填埋场中的微好氧和缺氧环境,探讨了甲烷氧化与反硝化的耦合作用,分析了相关微生物群落。结果表明:微好氧系统和缺氧系统的最大反硝化率分别为100%和85.67%,最大反硝化效率分别为16.54 mmol/(L·d)和9.88 mmol/(L·d);未通甲烷的对照系统中无反硝化现象,微好氧和缺氧系统均有良好的甲烷氧化耦合反硝化相关性;30%~40%二氧化碳的通入可使两个系统的pH分别稳定在7.6和7.4,对反硝化碱度累积有良好的缓冲作用;甲烷氧化的优势菌为甲基杆菌和甲基单胞菌,耦合的反硝化菌主要为甲基芽孢杆菌。In order to study the coupling relationship between methane oxidation and denitrification under different oxygen concentrations,three leaching bed bioreactors were used to simulate the microaerobic and anoxic environment in a semi-aerobic landfill.The coupling effect of methane oxidation and denitrification was discussed,and the related microbial communities were analyzed.The results showed that the maximum denitrification efficiencies of the microaerobic system and anoxic system were 100%and 85.67%,respectively.The maximum denitrification rates were 16.54 mmol/(L·d)and 9.88 mmol/(L·d),respectively.Denitrification process did not occur in the control system without methane.There was a good correlation between methane oxidation and denitrification in both microaerobic and anoxic systems.Introducing 30%~40%CO_(2) into the above two systems will stabilized their pH at 7.6 and 7.4,respectively,and had a good buffer effect on the accumulation of denitrification alkalinity.The dominant bacteria for methane oxidation were Methylobacter and Methylomonas,and the coupled denitrifying bacteria were mainly Methylobacillus.

关 键 词：准好氧填埋场 微氧甲烷氧化 缺氧甲烷氧化 渗滤液脱氮 微生物群落 

分 类 号：X703[环境科学与工程—环境工程]