木槿的化学成分研究  

Chemical Constituents from the Stems of Hibiscus syriacus

作　　者：程子洋[1] 柯仲成[1] 张愉快 赵奎奎 王国凯 CHENG Zi-yang;KE Zhong-cheng;ZHANG Yu-kuai;ZHAO Kui-kui;WANG Guo-kai(College of Chemistry and Chemical Engineering,Huangshan University,Huangshan 245041,Anhui,China;School of Pharmacy,Anhui University of Chinese Medicine,Hefei 230012,China)

机构地区：[1]黄山学院化学化工学院,安徽黄山245041 [2]安徽中医药大学药学院,合肥230012

出　　处：《热带亚热带植物学报》2021年第3期331-338,共8页Journal of Tropical and Subtropical Botany

基　　金：安徽省高校自然科学研究一般项目(KJHS2019B05);安徽省高校优秀青年人才支持计划重点项目(gxyqZD2019035);安徽省大学生创新创业项目(S201910375068)资助。

摘　　要：为了解木槿(Hibiscus syriacus)茎的化学成分,采用MCI-gel中压柱层析、硅胶柱层析、Sephadex LH-20凝胶柱层析和高效液相色谱等色谱方法,从木槿茎85%乙醇提取物中分离得到13个化合物。根据理化性质和波谱数据,其结构分别鉴定为methyl 4-[2-hydroxy-2-(4-hydroxy-3-methoxyphenyl)-1-(hydroxymethyl)ethyl]sinapate(1)、2,6,2′,6′-四甲氧基-4,4′-二(2,3-环氧-1-羟基丙基)二苯(2)、3,4,5-三甲氧基肉桂酸甲酯(3)、3,4-二甲氧基肉桂酸甲酯(4)、对羟基肉桂酸甲酯(5)、咖啡酸甲酯(6)、阿魏酸甲酯(7)、丁香脂素(8)、clemaphenol A(9)、(E)-3-hydroxyanetholeβ-D-glucopyranoside(10)、荭草苷(11)、木犀草素(12)和芹菜素(13)。其中化合物1~10为木脂素类,化合物11~13为黄酮类,化合物1为新天然产物,化合物1~12为首次从该植物中分离得到。采用MTS法检测,40μmol/L的化合物1~10对人白血病HL-60细胞和人肺癌A-549细胞株体外生长均未显示出较好的抑制活性。In order to understand the chemical constituents of Hibiscus syriacus stem,thirteen copounds were isolated from 85%ethanol extract of its stems by several column chromatographic techniques,such as MPLCMCI-gel,silica gel,Sephadex LH-20 and preparative HPLC.Based on physicochemical properties and NMR spectral data,their structures were identified as methyl 4-[2-hydroxy-2-(4-hydroxy-3-methoxyphenyl)-1-(hydroxymethyl)ethyl]sinapate(1),2,6,2?,6?-tetramethoxy-4,4?-bis(2,3-epoxy-1-hydroxypropyl)biphenyl(2),3,4,5-trimethoxycinnamic acid methyl ester(3),3,4-dimethoxycinnamic acid methyl ester(4),trans-p-hydroxycinnamic acid methyl ester(5),methyl caffeate(6),methyl feruate(7),syringaresinol(8),clemaphenol A(9),(E)-2-methoxy-5-propenylphenylβ-D-glucopyranoside(10),orientin(11),luteolin(12),and apigenin(13).Among them,compounds 1-10 were lignans and compounds 11-13 were flavonoids.Compound 1 was a new natural product,compound 1-12 were isolated from this plant for the first time.Compounds 1-10 at 40μmol/L did not exhibit good cytotoxic activities to in vitro growth of human leukemia HL-60 cells and human lung cancer A-549 cells.

关 键 词：木槿 化学成分 木脂素 结构鉴定 

分 类 号：R284.1[医药卫生—中药学]