羟基化氮化硼纳米管的表征及其对L02细胞的影响  

Characterization of hydroxylated boron nitride nanotubes and its effect on L02 cells

作　　者：杨欢 李崴[1] 许蒙 陈拥军[1] 骆丽杰[1] YANG Huan;LI Wei;XU Meng;CHEN Yongjun;LUO Lijie(School of Materials Science and Engineering,Hainan University,Haikou 570228,Hainan,China)

机构地区：[1]海南大学材料科学与工程学院,海南海口570228

出　　处：《精细化工》2021年第5期941-946,共6页Fine Chemicals

基　　金：2019年海南省基础与应用基础研究计划(自然科学领域)高层次人才项目基金(2019RC029);国家自然科学基金(20121707);海南省自然科学基金(518MS021)。

摘　　要：采用HCl溶液和HNO_(3)溶液对氮化硼纳米管(BNNTs)进行纯化和氧化,制备了羟基化的BNNTs(BNNTs-OH)。采用XPS、FTIR、TG、TEM、Zeta电位和PL对BNNTs-OH的形貌、结构和性能进行了表征。结果表明,BNNTs被成功氧化并截短,在B位点引入了—OH。BNNTs-OH在水溶液中具有很好的分散性,平均水合粒径约为1246.7 nm。BNNTs-OH具有特殊的荧光特性,在372 nm激发波长下可以发红色的光,实现材料在细胞内的定位。细胞毒性测试表明,BNNTs-OH对人胚胎肝细胞系L02细胞未呈现毒性。Hydroxylated boron nitride nanotubes(BNNTs-OH) were prepared by purification and oxidation of boron nitride nanotubes(BNNTs) with hydrochloric acid solution and nitric acid solution. The morphology, structure and properties of BNNTs-OH were characterized by XPS, FTIR, TG, TEM, Zeta potential and photoluminescence spectrum. The results showed that BNNTs were successfully oxidized and hydroxylation occurred at the B site. BNNTs-OH had good dispersibility in aqueous solution with an average hydration substitution of about 1246.7 nm. BNNTS-OH exhibited special fluorescence properties, and could emit red light at 372 nm to realize the location of BNNTs-OH in cells. Cytotoxicity tests indicated that BNNTs-OH had no cytotoxicity on L02 cells, a human embryonic liver cell line.

关 键 词：氮化硼纳米管 羟基化 表征 生物相容性 L02细胞 功能材料 

分 类 号：R318.08[医药卫生—生物医学工程] TB383[医药卫生—基础医学]