Tb^(3+)修饰的MOF荧光探针的制备及L-色氨酸识别性能  

Preparation and L-Tryptophan sensing performance of Tb^(3+) decorated fluorescent MOF

作　　者：高新丽 裴雷 赵旭东 高竹青[2] 黄宏亮 GAO Xin-li;PEI Lei;ZHAO Xu-dong;GAO Zhu-qing;HUANG Hong-liang(Instrumental Analysis Center,Taiyuan University of Science and Technology,Taiyuan 030024,China;College of Chemical and Biological Engineering,Taiyuan University of Science and Technology,Taiyuan 030024,China;School of Chemistry and Chemical Engineering,Tiangong University,Tianjin 300387,China;State Key Laboratory of Separation Membranes and Membrane Processes,Tiangong University,Tianjin 300387,China)

机构地区：[1]太原科技大学分析测试中心,太原030024 [2]太原科技大学化学与生物工程学院,太原030024 [3]天津工业大学化学与化工学院,天津300387 [4]天津工业大学省部共建天津工业大学分离膜和膜过程国家重点实验室,天津300387

出　　处：《材料工程》2021年第6期148-155,共8页Journal of Materials Engineering

基　　金：国家自然科学基金(21978212);山西省高校科技创新项目(2019L0628)。

摘　　要：以金属-有机骨架材料UiO-66-(COOH)_(2)为基底,采用后修饰法负载Tb^(3+)制备了荧光材料Tb^(3+)@UiO-66-(COOH)_(2),用于选择性检测色氨酸。利用透射电子显微镜(TEM)、X射线粉末衍射仪(XRD)、比表面积分析仪、红外光谱仪(FT-IR)、X射线光谱仪(XPS)等表征方法分析多孔材料的形貌、成分、孔道特性,利用紫外/可见分光光度计(UV-Vis)和荧光光谱仪研究材料的发光特性及氨基酸识别性能。结果表明:MOF材料中配体向Tb^(3+)的能量转移,诱导材料发出明亮的绿色荧光。在13种氨基酸中,只有色氨酸(Trp)对Tb^(3+)@UiO-66-(COOH)_(2)具有明显的荧光淬灭效应,这是由于色氨酸的紫外吸收峰和Tb^(3+)@UiO-66-(COOH)_(2)的荧光激发光谱有较大重合,表明二者对紫外光的吸收具有竞争性,削弱了Tb^(3+)@UiO-66-(COOH)_(2)的紫外光吸收,从而降低了材料的发光强度。进一步发现该材料可灵敏地检测色氨酸(检测限5.53μmol/L),同时具有良好的抵抗来自其他氨基酸干扰的能力。因此,本工作证明了Tb^(3+)@UiO-66-(COOH)_(2)可作为选择性检测色氨酸的荧光探针,同时也为未来色氨酸荧光探针的设计提供了一定的借鉴。For selectively sensing Tryptophan(Trp),the fluorescent Tb^(3+)@UiO-66-(COOH)_(2) was prepared through loading Tb^(3+) into UiO-66-(COOH)_(2) via post-synthesis method.Transmission electron microscope (TEM),X-ray powder diffractometer (XRD),specific surface area analyzer,and X-ray photoelectron spectroscope (XPS) were used to analysize the morphology,composition and pore characteristics of the porous materials.Meanwhile,ultraviolet-visible spectrophotometer(UV-Vis)and fluorescence spectrophotometer were used to study the fluorescence characteristics.The results show that the energy transfer from organic ligand to Tb^(3+) leads to a bright green fluorescence.Among the 13 amino acids,only Tryptophan has obvious fluorescence quenching effect.This is because there appears a large overlap between UV-Vis absorption spectrum of Trp and excitation spectrum of the MOF,which indicates a strong competition effect for UV light.The absorption to UV light of Tb^(3+)@UiO-66-(COOH)_(2) is decreased by Trp,leading to the weak fluorescence.Further investigation indicates the material can sensitively sense Trp(LOD,5.53μmol/L)and owns excellent anti-interference ability from other co-existing amino acids.Thus,this work not only demonstrates Tb^(3+)@UiO-66-(COOH)_(2) can serve as a selective and sensitive sensor for Trp,but also provides a guideline for designing novel sensors for amino acids in the future.

关 键 词：金属-有机骨架材料 后合成修饰法 色氨酸 荧光淬灭 

分 类 号：O482.31[理学—固体物理]