A rice tubulin tyrosine ligase-like 12 protein affects the dynamic and orientation of microtubules  

作　　者：Kunxi Zhang Xin Zhu Steffen Durst Petra Hohenberger Min‐Jung Han Gynheung An Vaidurya PSahi Michael Riemann Peter Nick 

机构地区：[1]Molecular Cell Biology,Botanical Institute,Karlsruhe Institute of Technology,Karlsruhe 76131,Germany [2]Aptamer Initiative,Postech Biotech Center,Pohang University of Science and Technology,Pohang‐si,Gyeongsangbuk‐do,37673,South Korea [3]Department of Plant Molecular Systems Biotech,Kyung Hee University,Yongin 446‐701,South Korea

出　　处：《Journal of Integrative Plant Biology》2021年第5期848-864,共17页植物学报（英文版）

基　　金：This work was supported by a fellowship from the the Chinese Scholarship Council to Kunxi Zhang;Open access funding enabled and organized by Projekt DEAL。

摘　　要：The detyrosination/retyrosination cycle is the most common post-translational modification of α-tubulin.Removal of the conserved C-terminal tyrosine of α-tubulin by a still elusive tubulin tyrosine carboxypeptidase, and religation of this tyrosine by a tubulin tyrosine ligase(TTL), are probably common to all eukaryotes. Interestingly, for plants, the only candidates qualifying as potential TTL homologs are the tubulin tyrosine ligase-like 12 proteins. To get insight into the biological functions of these potential TTL homologs, we cloned the rice TTL-like 12 protein(Os TTLL12)andgeneratedoverexpression Os TTLL12-RFP lines in both rice and tobacco BY-2 cells. We found, unexpectedly, that overexpression of this Os TTLL12-RFP increased the relative abundance of detyrosinated α-tubulin in both coleoptile and seminal root, correlated with more stable microtubules. This was independent of the respective orientation of cortical microtubule, and followed by correspondingly changing growth of coleoptiles and seminal roots. A perturbed organization of phragmoplast microtubules and disoriented cell walls were further characteristics of this phenotype. Thus, the elevated tubulin detyrosination in consequence of Os TTLL12 overexpression affects structural and dynamic features of microtubules, followed by changes in the axiality of cell plate deposition and, consequently, plant growth.

关 键 词：microtubule PHRAGMOPLAST RICE tobacco BY-2 tubulin detyrosination tubulin tyrosine carboxypeptidase tubulin tyrosine ligase tubulin tyrosine ligase-like 12 

分 类 号：S511[农业科学—作物学]