外源24-表油菜素内酯对NaCl胁迫下桑树幼苗的缓解效应  

Alleviation Effect of Exogenous 24-Epigenolide on Mulberry Seedlings Under NaCl Stress

作　　者：董亚茹[1] 张艳波 赵东晓[1] 耿兵 娄齐年[1] 李云芝[1] 王照红[1] 郭光[1] DONG Yaru;ZHANG Yanbo;ZHAO Dongxiao;GENG Bing;LOU Qinian;LI Yunzhi;WANG Zhaohong;GUO Guang(Shandong Sericulture Research Institute,Yantai,Shandong 264002)

机构地区：[1]山东省蚕业研究所,山东烟台264002

出　　处：《核农学报》2021年第6期1466-1475,共10页Journal of Nuclear Agricultural Sciences

基　　金：山东省自然科学基金项目(ZR2019PC043);山东省现代农业产业技术体系蚕桑产业创新团队(SDAIT-18-02、SDAIT-18-01);烟台市科技创新发展计划(2021YT06000724);山东省科技特派员行动计划(2020KJTPY070)。

摘　　要：为探究施加外源24-表油菜素内酯(EBR)对桑树耐盐性的影响,以桑品种桂桑优12为材料进行盆栽试验,在200 mmol·L^(-1) NaCl胁迫下,研究外施不同浓度(0.01、0.10、1.00μmol·L^(-1))EBR对盐胁迫下桑树幼苗质量、根系特征、光合色素含量、活性氧(ROS)水平、丙二醛(MDA)含量、相对电导率、抗氧化酶活性和渗透调节物质含量等生理指标的影响。结果表明,与单一NaCl胁迫相比,外源添加0.10μmol·L^(-1)EBR后,桑树幼苗地上鲜质量和干质量、主根长、总根长、根表面积、根平均直径、根总体积、叶绿素和类胡萝卜素含量均提高,叶片与根中产生羟基自由基(·OH)能力、产生超氧阴离子自由基(O_(2))活力、过氧化氢(H_(2)O_(2))含量降低;叶片与根中的超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性升高;脯氨酸、可溶性蛋白和可溶性糖含量增加;相对电导率和MDA含量降低。其中地下部鲜质量和根体积是盐胁迫下的1.56和1.57倍,叶片中CAT活性和可溶性糖含量达到盐胁迫下的1.57和1.68倍。本研究为揭示外施油菜素内酯增强桑树耐盐性的生理调控机制提供了参考依据。In order to explore the effect of exogenously 2, 4-epibrassinolide(EBR) on the salt tolerance of mulberry(Morus L.), a pot experiment was carried out with mulberry variety gui sang you 12 as the material. Different concentrations(0.01, 0.10, 1.00μmol·L^(-1)) of exogenous EBR were applied to investigate the effects of exogenous EBR on quality, root characteristics, pigment content, reactive oxygen species(ROS) level, malondialdehyde(MDA) content, relative permeability of plasma membrane, antioxidant enzyme activity and osmotic regulator content of mulberry seedlings under 200 mmol·L^(-1) NaCl stress. The results showed that the shoot fresh mass and dry mass, root fresh and dry mass, taproot length, total root length, root surface area, root average diameter, total root volume and the content of chlorophyll and carotenoids of mulbery seedling were increased when 0.10 μmol·L^(-1)EBR was applied under salt stress. The ability to produce hydroxyl(·OH) and superoxide radicals(O_(2)) in leaves and roots were decreased, as well as the content of hydrogen peroxide(H_(2)O_(2)). On the other hand, the activities of superoxide dismutase(SOD), peroxidase oxidase(POD) and catalase(CAT) were increased. Meanwhile, the contents of proline, soluble protein and soluble sugar increased, but relative permeability of plasma membrane and MDA content dropped down. Among them, the root fresh mass and root volume were 1.56 and 1.57 times of salt stress, and the CAT activity and soluble sugar content in leaves reached 1.57 and 1.68 times of salt stress, respectively. This study provides a reference for revealing the physiological regulation mechanism of the external application of EBR to enhance the salt tolerance of mulberry.

关 键 词：油菜素内酯 桑树 NACL胁迫 生理特性 

分 类 号：S888.2[农业科学—特种经济动物饲养]