荧光激活细胞分选技术加速酶定向进化  

Accelerating directed evolution of enzyme by fluorescence-activated cell sorting

作　　者：朱润涛 高浩峰[1] 杨一帆 刘永琪 李佳祺 张霞[1] 胡南[1] ZHU Runtao;GAO Haofeng;YANG Yifan;LIU Yongqi;LI Jiaqi;ZHANG Xia;HU Nan(Colloge of Biotechnology and Pharmaceutical Engineering,Nanjing Tech University,Nanjing 211800,China)

机构地区：[1]南京工业大学生物与制药工程学院,江苏南京211800

出　　处：《生物加工过程》2021年第3期234-240,共7页Chinese Journal of Bioprocess Engineering

基　　金：江苏先进生物与化学制造协同创新中心自主研发课题(XTD1824)。

摘　　要：目前,定向进化技术是改造工业酶的主要方法,而高通量筛选技术是酶定向进化的瓶颈技术之一。其中,荧光激活细胞分选技术(FACS)因其具有极高的筛选效率和广阔的通用性逐渐成为酶定向进化中首选的高通量筛选技术。依据不同的区室化策略,将荧光激活细胞分选技术分成细胞、液滴和微室高通量筛选技术。本文综述这3种新兴的筛选方法在技术方面的优劣势,并展望荧光激活细胞分选技术在未来的应用前景。Directed evolution is an mportant method to engineer industrial enzymes although high-throughput screening(HTS)is the major bottleneck.Fluorescence-activated cell sorting(FACS)has gradually become the preferred high-throughput screening technology in enzyme directed evolution due to its higher screening efficiency and wider versatility.By using varies compartmentalization technologies,FACS can be divided into cell-,droplet-and microchamber-based platforms for HTS technologies.Here,we review benefits and limitations of each emerging platform,and indicate the application prospect of FACS.

关 键 词：定向进化 高通量筛选技术 荧光激活分选技术 区室化策略 

分 类 号：Q816[生物学—生物工程]