中华绒螯蟹四倍体诱导研究  

Tetraploidy Induction to Eriocheir sinensis(Milne, Edwards)

作　　者：崔朝霞[1] 相建海[1] 周岭华[1] 

机构地区：[1]中国科学院海洋研究所实验海洋生物学重点研究实验室,青岛266071

出　　处：《高技术通讯》2002年第12期97-102,共6页Chinese High Technology Letters

基　　金：86 3计划 ( 86 3D0 196 30 5 1);瑞典国际基金 (IFSA/32 2 5 - 1)资助项目。

摘　　要：分别使用C B、6 DMAP和中科院海洋所专有诱导剂PA三种试剂诱导中华绒螯蟹 (Eriocheirsinensis)四倍体 ,囊胚期获得的最高诱导率依次是 5 0 31%、5 4 87%和79 75 %。在使用PA作为诱导剂的实验中 ,于受精卵排出后 2 0h、浓度为 5 0倍稀释液、处理持续时间 2 4 0min ,囊胚期检测 ,最高诱导率 85 60 %。将抱卵蟹浸入PA液进行处理 ,受处理卵培育至蚤状幼体检测 ,最高诱导率 2 7 2 9%。The incubating temperature was set at 18℃ to induce the tetraploidy Eriocheir sinensis through inhibition of the first cleavage. Flow cytometry was used to identify the ploidy on the different development stages. In the tetraploid fertilized eggs induction, the highest induction rate by C.B, 6-DMAP and PA are 50.31%, 54.87% and 79.75% respectively in blastula. With PA(50 times diluted solution) as inducer at 20 hour after ovulation, and treatment time being 240min, the highest induction rate is 85.60% in blastula. In the PA treatment of pregnant crabs with fertilized eggs, the experiment results are as follows: the highest induction rate are 80.18% in gastrula and 27.29% in zoea.

关 键 词：诱导 中华绒螯蟹 四倍体 蚤状幼体 水产养殖 

分 类 号：S966.16[农业科学—水产养殖]