高盐度环境下某污水处理厂AAO生化系统微生物群落变化分析  

ANALYSIS ON CHANGE OF MICROBIAL COMMUNITY IN AAO BIOCHEMICAL SYSTEM OF A SEWAGE TREATMENT PLANT UNDER HIGH SALINITY ENVIRONMENT

作　　者：殷豪帅 黄开 王卿卿 李世峰 YIN Hao-shuai;HUANG Kai;WANG Qing-qing;LI Shi-feng(School of Environmental and Municipal Engineering,Qingdao University of Technology,Qingdao 266033,China;Shanghai Municipal Engineering Design Institute(Group)Co.,Ltd,Shanghai 200092,China)

机构地区：[1]青岛理工大学环境与市政工程学院,山东青岛266033 [2]上海市政工程设计研究总院(集团)有限公司,上海200092

出　　处：《环境工程》2021年第3期68-74,共7页Environmental Engineering

摘　　要：为研究高盐度环境下AAO生化系统的活性污泥优势微生物,利用高通量测序技术对不同时期污泥进行微生物结构进行分析。结果表明:ρ(Cl^(-))从1000 mg/L上升到5000 mg/L的过程中,微生物群落结构发生明显变化。持久型OTUs占总序列90.59%,其中Proteobacteria(变形菌门)相对丰度始终在40%以上,Chiorobi(绿菌门)相对丰度从6.11%上升至16.13%。微生物属水平分析发现,Methyloceanibacter(16.94%~27.44%)是高盐条件下主要的有机物去除菌属,Ignavibacterium(18.43%~26.78%)是主要除硫菌属,Dechloromonas(1.52%~3.05%)、Nitrospirae(1.9%~8.84%)、Nitrosomonas(1%左右)是主要的脱氮菌属。In order to study the dominant microorganisms of activated sludge in AAO biochemical system at high Cl^(-)concentration,high-throughput sequencing technology was used to analyze the microbial structure of sludge in different periods.The results showed that the microbial community structure changed obviously when the Cl^(-)concentration increased from 1000 mg/L to 5000 mg/L.Persistent OTUs accounted for 90.59%of the total sequence.The relative abundance of Proteobacteria in the bacterial community was stable at 40%above.The relative abundance of Chiorobi increased from 6.11%to 16.13%.Generic level analysis of microorganisms showed that Methyloceanibacter(16.94%~27.44%)was the main organic matter removal bacteria under the condition of high salinity.Ignavibacterium(18.43%~26.78%)played an important role in the removal of sulfides.Dechloromonas(1.52%~3.05%),Nitrospirae(1.9%~8.84%)and Nitrosomonas(about 1%)was the main bacteria to remove nitrogen pollutants.

关 键 词：高盐度 高通量测序 微生物群落变化 活性污泥 

分 类 号：X703[环境科学与工程—环境工程]