奇岗微繁技术建立及幼苗耐盐性评价  

A micropropagation system for Miscanthus×giganteus based on axillary buds and evaluation of its salt tolerance

作　　者：王晔[1] 陈慧萍 李润枝[1] 彭真 范希峰[3] 武菊英[3] 段留生 Ye WANG;Hui-ping CHEN;Run-zhi LI;Zhen PENG;Xi-feng FAN;Ju-ying WU;Liu-sheng DUAN(College of Plant Science and Technology,Beijing University of Agriculture,Beijing 102206,China;Engineering Research Center of Plant Growth Regulator,Ministry of Education,College of Agronomy,China Agricultural University,Beijing 100193,China;Beijing Research and Development Center for Grass and Environment,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097,China)

机构地区：[1]北京农学院,植物科学技术学院,北京102206 [2]中国农业大学农学院,植物生长调节剂教育部工程研究中心,北京100193 [3]北京市农林科学院,北京草业与环境研究发展中心,北京100097

出　　处：《草业学报》2021年第6期214-220,共7页Acta Prataculturae Sinica

基　　金：北京市科技计划课题(D171100007217001)资助

摘　　要：以奇岗茎段作为外植体,确定了最佳取材时期、诱导培养基、生根培养基和分化培养基等,成功建立了奇岗微繁技术体系,并研究了不同浓度NaCl对微繁苗生长速率和生物量的影响。结果表明:腋芽生枝途径微繁体系以5~6叶期茎段为外植体,在75%酒精喷洒表面后,1.0%次氯酸钠浸泡20 min时,污染率为0且出芽诱导率较高(85.2%)。当诱导和继代的MS培养基添加5.0 mg·L^(-1)6-苄氨基嘌呤(6-BA)和0.25 mg·L^(-1)萘乙酸(NAA)时,出芽诱导率为84.0%,繁殖系数最高(320.3%)。生根培养基添加5.0 g·L^(-1)活性炭适合于生根壮苗培养。将试管苗炼苗后移栽到不同NaCl浓度的培养液中,随着NaCl的浓度增加,微繁苗生长速率呈降低趋势;在低于0.8%NaCl条件下奇岗能正常成活,表明微繁苗具有较高的耐盐性。为奇岗快繁、遗传转化体系构建和在盐渍化土地推广应用提供了理论和技术支持。The aim of this study was to develop a micropropagation system for Miscanthus×giganteus and to evaluate its salt tolerance.We used nodal segments as explants and determined the optimum developmental stage to collect explants,the best culture medium for axillary bud induction,and the conditions for differentiation of callus and roots.Then,we established a regeneration system via tissue culture.The tissue culture system was based on the axillary shoot growth pathway.The best results were obtained by collecting nodal segments about 15 mm in length from plants at the 5-6 leaf stage,and sterilizing them by surface-spraying with 75%ethanol followed by immersion in 1.0%sodium hypochlorite for 20 min.Under these conditions,the pollution rate was zero and the adventitious bud induction rate was 85.2%.The most suitable medium for induction and subculturing of callus was Murashige and Skoog(MS)medium containing 5.0 mg?L-16-benzylaminopurine and 0.25 mg?L-11-naphthaleneacetic acid;the multiplication rate on this medium was 320.3%.For rooting and growth of cluster buds,the most suitable medium was MS medium containing 5.0 g?L-1 activated carbon.The salt tolerance of Miscanthus×giganteus plantlets was evaluated under hydroponic culture conditions.Increasing concentrations of NaCl in the hydroponic culture medium led to decreased growth rate of the plantlets.The plantlets were able to survive normally in medium containing 0.8%NaCl in the hydroponic system,indicative of high salt tolerance.These results provide a theoretical and technical basis for the rapid propagation of Miscanthus×giganteus that can be further developed for genetic transformation and the production of Miscanthus×giganteus lines for cultivation in salinized areas.

关 键 词：奇岗 茎段 微繁 耐盐性 

分 类 号：S578[农业科学—作物学]