微藻胞间植物激素甄别方法与研究进展  

Identification of plant hormones in intercellular microalgae cells and prospects of future studies

作　　者：彭石子 陈从立 李小双 周奕含[2] 王建伟[2] 周丹丹[1] PENG Shizi;CHEN Congli;LI Xiaoshuang;ZHOU Yihan;WANG Jianwei;ZHOU Dandan(Engineering Laboratory for Water Pollution Control and Resources Recovery in Jilin Province,Northeast Normal University,Changchun,Jilin 130000,China;National Analytical Research Center of Electrochemistry and Spectroscopy,Changchun Institute of Applied Chemistry Chinese Academy of Sciences,Changchun,Jilin 130022,China)

机构地区：[1]东北师范大学吉林省水污染控制与资源化工程实验室,吉林长春130000 [2]中国科学院长春应用化学研究所国家电化学和光谱研究分析中心,吉林长春130022

出　　处：《微生物学通报》2021年第5期1689-1702,共14页Microbiology China

基　　金：国家自然科学基金(51722803,52070036);中央高校基本科研业务费专项资金(2412018ZD042)。

摘　　要：植物激素是一类微量的内源化合物,可能是微藻生命活动过程中的通用“语言”。植物激素甄别是认知微藻群体感应和胞间通讯机制的关键。然而,由于植物激素具有超微量、性质复杂和干扰物质共存的特点,选择适宜的提取浓缩方法、提高检测灵敏度、减小基质效应和区分同分异构体仍是甄别植物激素的难点问题。本文综述了植物激素提取与检测的研究现状,关注了各类样品制备植物激素的方法和案例,重点阐释了固相萃取法、液液萃取法、磁性固相萃取法、液液微萃取法等提取方法以及液相色谱、液相色谱串联质谱、毛细管电泳等检测方法的优缺点,展望了利用串联固相萃取法从藻液提取痕量植物激素的前景,以及采用超高效液相色谱-线性离子阱-静电场轨道阱组合式高分辨质谱检测植物激素的方法,以期为藻际微生物生态相关研究提供方法参考。Plant hormones is one of trace endogenous compounds,which probably are the universal“languages”among microalgae cells.The screening and identification of plant hormones play a significant role in understanding the quorum sensing and intercellular communication mechanisms of microalgae cells.However,these hormones are usually in ultra-trace levels,with various species,complicate properties,and coexist with complex matrices.Thus,it is still very challenging to develop an effective method to identify the plant hormones,which includes verifying extraction procedures,improving detection sensitivity,reducing matrix effects and distinguishing isomers.In this work,we reviewed the present research status of plant hormones extraction and detection,especially focused on the methods and strategies on preparing plant hormones from various samples.The advantages and disadvantages of these pretreatment methods were systematically elaborated,from aspects of solid phase extraction,liquid extraction,magnetic solid phase extraction,liquid-liquid microextraction,etc.The detection methods,such as liquid chromatography,liquid chromatography tandem mass spectrometry,capillary electrophoresis,etc.,were also compared.Then,we look forward to the prospects of tandem solid phase extraction method on extracting trace amounts of plant hormones from algae slurry.We considered that UPLC-LTQ-Orbitrap-MS method would be a promising technology for plant hormones detection.We aimed to provide some method references for the study of inter-algal microbial ecology.

关 键 词：微藻 植物激素 萃取 甄别 液质联用 

分 类 号：Q946.885[生物学—植物学]