2型糖尿病肾病患者血清miR-92b-5p和HMGB1水平变化及临床意义  

Changes and clinical significance of miR-92b-5p and HMGB1 in serum of patients with type 2 diabetic nephropathy

作　　者：陈晨[1] 李晶[1] 叶婷[1] 李惠[1] CHEN Chen;LI Jing;YE Ting;LI Hui(Traditional Chinese Medicine Hospital Affiliated to Xinjiang Medical University,Urumqi 830000,China)

机构地区：[1]新疆医科大学附属中医医院,乌鲁木齐830000

出　　处：《山东医药》2021年第16期6-10,共5页Shandong Medical Journal

基　　金：新疆维吾尔自治区自然科学基金资助项目(2020D01C133)。

摘　　要：目的观察miR-92b-5p及高迁移率族蛋白Box1(HMGB1)在2型糖尿病肾病患者血清中的水平变化,并探讨其临床意义。方法选取2型糖尿病患者203例,依据是否合并肾病将患者分为单纯糖尿病组(n=98)和糖尿病肾病组(n=105),并纳入80例同期体检健康者为对照组。采用酶联免疫吸附法检测各组血清HMGB1,采用实时荧光定量PCR法检测各组血清miR-92b-5p,采用多因素Logistic回归分析2型糖尿病肾病的危险因素,采用Pearson检验进行相关性分析,采用ROC曲线评估miR-92b-5p和HMGB1对2型糖尿病肾病的预测价值。结果单纯糖尿病组和糖尿病肾病组血清HMGB1水平高于对照组,且糖尿病肾病组高于单纯糖尿病组(P均<0.05)。单纯糖尿病组和糖尿病肾病组血清miR-92b-5p水平低于对照组,且糖尿病肾病组低于单纯糖尿病组(P均<0.05)。多因素Logistic回归分析显示,血清HMGB1水平升高与miR-92b-5p水平降低均是2型糖尿病进展为2型糖尿病肾病的独立危险因素(P均<0.05)。2型糖尿病患者血清miR-92b-5p水平与HMGB1和24 h尿蛋白(24 hpro)水平均呈负相关,与估算肾小球滤过率(eGFR)水平呈正相关(P均<0.05);HMGB1和24 hpro水平呈正相关,与eGFR水平呈负相关(P均<0.05)。血清HMGB1联合miR-92b-5p预测2型糖尿病肾病的ROC曲线下面积(AUC)为0.858(95%CI:0.800~0.908),高于miR-92b-5p(AUC=0.754,95%CI:0.688~0.821)、HMGB1(AUC=0.802,95%CI:0.737~0.860)单一检查(P均<0.05)。结论血清HMGB1水平升高和miR-92b-5p水平降低是2型糖尿病患者进展为糖尿病肾病的危险因素,早期联合检测两指标可作为临床预测及诊断2型糖尿病肾病的重要生化标志物。Objective To observe the changes of miR-92b-5p and high-mobility histone Box1(HMGB1)in serum of patients with type 2 diabetic nephropathy and to explore its clinical significance.Methods Two hundred and three patients with type 2 diabetes mellitus were selected and divided into the simple diabetes group(n=98)and the diabetic nephropathy group(n=105)according to whether they were complicated with nephropathy.Eighty healthy people in the same period were included as the control group.Enzyme linked immunosorbent assay(ELISA)was used to detect serum HMGB1,real-time PCR was used to detect serum mir-92b-5p,multivariate Logistic regression was used to analyze the risk factors of type 2 diabetic nephropathy,Pearson test was used for correlation analysis,and ROC curve was used to evaluate the predictive value of miR-92b-5p and HMGB1 for type 2 diabetic nephropathy.Results The levels of serum HMGB1 in the simple diabetes group and the diabetic nephropathy group were higher than those in the control group,and the level in the diabetic nephropathy group was higher than that in the simple diabetes group(all P<0.05).The levels of miR-92b-5p in the simple diabetes group and the diabetic nephropathy group were lower than those in the control group,and the level of miR-92b-5p in the diabetic nephropathy group was lower than that in the simple diabetes group(all P<0.05).Multivariate Logistic regression analysis showed that the increase of serum HMGB1 level and the decrease of miR-92b-5p level were independent risk factors in the progression of type 2 diabetes mellitus to type 2 diabetes nephropathy(all P<0.05).The level of serum miR-92b-5p was negatively correlated with serum HMGB1 and 24 h urinary protein(24 hpro),and positively correlated with estimated glomerular filtration rate(eGFR)in patients with type 2 diabetes mellitus(all P<0.05).Serum HMGB1 was positively correlated with 24 hpro,and negatively correlated with eGFR(all P<0.05).The area under the ROC curve(AUC)of serum HMGB1 combined with miR-92b-5p in predicting type 2 d

关 键 词：2型糖尿病肾病 微小RNA 92b-5p 高迁移率族蛋白Box1 尿蛋白 肾小球滤过率 

分 类 号：R587.2[医药卫生—内分泌]