26例病毒性脑(膜)炎儿童脑脊液病原学特征研究  

Pathogenic Characteristics of Cerebrospinal Fluid Among 26 Children with Viral Encephalitis and Meningitis

作　　者：朱以敏 刘静[2] 刘丽丽 李意杰 阚慧 李亚欣 张文[5] 陆国平[2] 郑英杰 ZHU Yimin;LIU Jing;LTU Lili;LI Yijie;KAN Hui;LI Yaxin;ZHANG Wen;LU Guoping;ZHENG Yingiie(Department of Epidemiology,School of Public Health,Fudan University,Shanghai 200032,China;Department of Crincal Care Medicine,Fudan University Affliated Pediatric Hospital,Shanghai200032,China;Key Laboratory for Health Technology Assessment,National Commission of Healh and Famnily Planning,Fudan Untversity,Shanghai200032,China;Key Laboratory of Public Health Safety,Ministry of Educaton,School of Public Health,Fudan Untversity,Shanghai 200032;School of Basic Medicine,Jiangsu Unitversity,Zhenjiang 212013,China)

机构地区：[1]复旦大学公共卫生学院流行病学教研室,上海200032 [2]国家卫生健康委员会卫生技术评估重点实验室(复旦大学),上海200032 [3]复旦大学公共卫生学院公共卫生安全教育部重点实验室,上海200032 [4]复旦大学附属儿科医院重症医学科,上海200032 [5]江苏大学基础医学院,镇江212013

出　　处：《病毒学报》2021年第3期583-590,共8页Chinese Journal of Virology

基　　金：国家重点研发计划“生物安全关键技术研发”重点专项(项目号:2017YFC1200203),题目:临床重症感染的宿主微生物组在发病与转归中的作用;国家自然科学基金(项目号:81373065),题目:母婴隐匿性乙型肝炎病毒感染及传播机制研究;国家自然科学基金(项目号:81773490),题目:基于队列的母婴微生物组与妊娠结局(早产)的关系研究。

摘　　要：病毒是儿童脑(膜)炎发病的重要病原体,为全面了解脑(膜)炎儿童脑脊液中的病毒感染情况,采集2019年6月-2019年9月复旦大学附属儿科医院26例病毒性脑(膜)炎(Viral encephalitis and meningitis,VEVM)儿童脑脊液标本,通过两种核酸检测方法进行病毒检测。病毒宏基因组测序检测到小RNA病毒科(Picornaviridae,82.42%)、浓病毒亚科(Densovirinae,9.07%)、指环病毒科(Anelloviridae,3.21%)、圆环病毒科(Circoviridae,0.85%)、星状病毒科(Astroviridae,0.76%)和小双节RNA病毒科(Picobirnaviridae,0.38%),通过聚合酶链式反应(Polymerase chain reaction,PCR)检测到肠道病毒(Enterovirus,EV)、单纯疱疹病毒1型(Herpes simplex virus,HSV-1)、人单纯疱疹病毒4型(Epstein-Barr virus,EBV)及腮腺炎病毒(Mumps virus,MuV),检出率分别为42.31%、7.69%、7.69%和3.85%。本研究初步明确了26例VEVM儿童脑脊液病毒信息,为深入研究其病原学进化分析奠定了基础。Viruses are the main pathogens in children suffering from encephalitis or meningitis.We wished to achieve comprehensive understanding of virus infection in the cerebrospinal fluid(CSF)of children with encephalitis and meningitis.Hence,26 specimens of CSF among children with viral encephalitis and viral meningitis(VEVM)were collected from June 2019 to September 2019 in the Children’s Hospital of Fudan University,China.Virus detection was conducted by two testing methods for nucleic acids.Viral metagenomics were used to detect Picornaviridae(82.42%),Densovirinae(9.07%),Anelloviridae(3.21%),Circoviridae(0.85%),Astroviridae(0.76%)and Picobirnaviridae(0.38%).Polymerase chain reactions were used to detect enteroviruses(EVs),Herpes simplex virus-1,Epstein-Barr virus and Mumps virus,and the detection rate was 42.31%,7.69%,7.69%and 3.85%,respectively.This study provides initial evidence of viral infection in CSF among 26 children with VEVM.Our study lays a foundation for further understanding of virus evolution.

关 键 词：病毒性脑(膜)炎 病毒宏基因组学 高通量测序 

分 类 号：R373[医药卫生—病原生物学]