内蒙古地区蒙古族儿童MPO基因与川崎病相关性研究  

Correlation between MPO Gene and Kawasaki Disease in Mongolian Children in Inner Mongolia

作　　者：肖蓉 常鑫 陈丽清 Xiao Rong;Chang Xin;Chen Liqing(Department of Paediatrics,Inner Mongolia Autonomous Region Maternal and Child Health Hospital,Inner Mogolia 010000,China)

机构地区：[1]内蒙古自治区妇幼保健院,呼和浩特010000

出　　处：《医学研究杂志》2021年第5期67-70,50,共5页Journal of Medical Research

基　　金：内蒙古自然科学基金资助项目(2017MS08128)。

摘　　要：目的探讨内蒙古地区蒙古族儿童髓过氧化物酶(myeloperoxidase,MPO)基因单核苷酸多态性(single nucleotide polymorphisms,SNP)rs2333227与川崎病易感性及其并发冠状动脉损害(coronary artery lesions,CAL)的关联性。方法采取病例对照研究,选取病例组100例典型川崎病蒙古族儿童及对照组100例正常体检的蒙古族儿童,根据有无累及冠状动脉,将病例组分为CAL组和NCAL组,采用聚合酶链反应(polymerase chain reaction,PCR)及直接测序法测定SNP位点多态性分布。结果病例组和对照组间MPO基因rs2333227位点(AA、GA、GG)基因型频率分布比较,差异无统计学意义(χ^(2)=2.392,P=0.302),其等位基因(A和G)频率分布比较差异无统计学意义(χ^(2)=1.592,P=0.351)。CAL组和NCAL组间该基因SNP位点的基因型频率分布比较差异有统计学意义(χ^(2)=6.263,P=0.044),其等位基因频率分布比较差异有统计学意义(χ^(2)=4.037,P=0.045),且GG基因型增加KD并发CAL的发生风险(OR=5.13,95%CI:1.22~21.63)。该基因位点基因型与川崎病典型临床表现与实验室相关结果(WBC、中性粒细胞百分比、红细胞沉降率、C反应蛋白(C reactive protein,CRP)及B型尿钠肽(brain natriuretic peptide,BNP))在基因型GG和GA+AA组间差异无统计学意义(P>0.05)。结论MPO基因rs2333227位点与内蒙古地区蒙古族儿童的川崎病并发CAL可能存在一定相关性,G等位基因为风险因子,与内蒙古地区蒙古族儿童患有川崎病的临床特点无明显相关性。Objective To study the association of single nucleotide polymorphism(SNP)rs2333227 of MPO gene with susceptibility to Kawasaki disease and Coronary artery lesions(CAL)in Mongolian children in Inner Mongolia.Methods A case-control study was conducted.100 typical Mongolian children with Kawasaki disease and 100 normal Mongolian children in the control group were selected.According to whether coronary artery was involved or not,and the case groups were divided into CAL group and NCAL group.Polymerase chain reaction(PCR)and direct sequencing method were used to determine SNP polymorphism distribution.Results There was no significant difference in genotype frequency distribution(χ^(2)=2.392,P=0.302)and allele frequency distribution(a and g)of MPO gene between case group and control group(χ^(2)=1.592,P=0.351).The genotype frequency distribution of SNP locus of this gene was significantly different between CAL group and NCAL group(χ^(2)=6.263,P=0.044),and the allele frequency distribution was significantly different(χ^(2)=4.037,P=0.045),and GG genotype increased the risk of KD complicated with CAL(OR=5.13,95%CI:1.22-21.63).There was no significant difference between genotype GG and GA+AA between the genotype GG and typical clinical manifestations of Kawasaki disease and laboratory related results[WBC,neutrophil percentage,erythrocyte sedimentation rate,C reactive protein(CRP)and brain natriuretic peptide(BNP)].Conclusion MPO gene rs2333227 may have a certain correlation with Kawasaki disease complicated by CAL in Mongolian children in Inner Mongolia.G allele is a risk factor,and there is no obvious correlation with the clinical features of Mongolian children in Inner Mongolia with Kawasaki disease.

关 键 词：川崎病 MPO基因 单核苷酸多态性 儿童 冠状动脉损害 

分 类 号：R725.4[医药卫生—儿科]