A Rapid Identification Method of Bactrocera cilifera (Hendel) with Species-Specific Primers(SS-COI)  

作　　者：Zhen Huang Qiongxia Guo 

机构地区：[1]College of Plant Protection,Fujian Agriculture and Forestry University,Fuzhou 350002,China [2]Fuzhou Changle Airport Customs,Fuzhou 50209,China [3]Fuzhou Customs Technical Center,Fuzhou 350001,China

出　　处：《Plant Diseases and Pests》2021年第2期33-37,共5页植物病虫害研究（英文版）

基　　金：Supported by Natural Science Foundation of Fujian Province (2011J01066, 2012JO1061)。

摘　　要：[Objective]The paper was to establish a rapid identification method of Bactrocera cilifera(Hendel)with species-specific primers(SS-COI).[Method]Using B.cilifera(Hendel)as the positive control,and 19 species of fruit flies such as B.diaphora(Coquillett)and B.dorsalis(Hendel)as the negative controls,a pair of species-specific primers,YF290 and YR511,were designed and screened for accurate identification of B.cilifera,based on mitochondrial DNA COI sequence.[Result]The PCR products were amplified and detected by electrophoresis.Only a clear and single band was observed at about 222 bp in the positive control,while no bands were found in the other negative controls.[Conclusion]The established rapid identification method with species-specific primers(SS-COI)is of great practical significance for rapid identification of fruit flies intercepted from import and export fruits and vegetables at ports,and for rapid clearance and early warning of import fruits and vegetables at ports.

关 键 词：Bactrocera cilifera(Hendel) Species-specific primers Species-specific PCR(SS-PCR) mt DNA COI Rapid identification 

分 类 号：S41-30[农业科学—植物保护]