基于中国部分地区无偿献血人群宏基因组学的新发病原体分析  

Emerging infectious diseases in voluntary blood donors in parts of China:Based on metagenomics analysis

作　　者：李昱辉 高瞻[1] 李世林[1] 李玉佳[1] 黄杨 陈利民[1] 黄梅[3] 万建华 何伟兰[5] 毛伟[6] 蔡杰[7] 周静宇[8] 杨茹 尹以靖 郭艳丽 何苗[1] LI Yu-hui;GAO Zhan;LI Shilin;LI Yujia;HUANG Yang;CHEN Limin;HUANG Mei;WAN Jianhua;HE Weilan;MAO Wei;CAI Jie;ZHOU Jingyu;YANG Ru;YIN Yijing;GUO Yanli;HE Miao(Institute of Blood Transfusion,Chinese Academy of Medical Sciences&Peking Union Medical College,Chengdu 610052,China;Sichuan Blood Safety and Blood Substitute,International Science and Technology Cooperation Base;The Red Cross Central Blood Station of Miarvyang;Blood Center of Urumqi;Blood Center of Guangxi Zhuang Autonomous Region;Blood Center of Chongqing;The Red Cross Blood Center of Nanjing;Blood Center of Jiangsu,Province;Blood Center of Wuhan;Central Blood Station of Dehong Prefecture;Central Blood Station of Mudanjiang)

机构地区：[1]中国医学科学院北京协和医学院输血研究所,四川成都610052 [2]四川血液安全和血液代用品国际科技合作基地 [3]绵阳市红十字中心血站 [4]乌鲁木齐血液中心 [5]广西壮族自治区血液中心 [6]重庆市血液中心 [7]南京红十字血液中心 [8]江苏省血液中心 [9]武汉血液中心 [10]德宏州中心血站 [11]牡丹江市中心血站

出　　处：《中国输血杂志》2021年第5期440-446,共7页Chinese Journal of Blood Transfusion

基　　金：中国医学科学院医学与健康科技创新工程(CAMS-2016-I2M-3-025)。

摘　　要：目的分析中国无偿献血者宏基因组学及微生物组学,评估新发病原体对输血安全的潜在威胁。方法收集2012~2018年重庆市、广西壮族自治区柳州市、新疆维吾尔自治区乌鲁木齐市、四川省绵阳市、湖北省武汉市、江苏省南京市、黑龙江省牡丹江市以及云南省德宏傣族景颇族自治州8个地区血站无偿献血者血浆标本12300(人)份(10 mL/份),以160份血样(0.1 mL/份)混合为1个单位做超速离心(32000 r/min,离心半径91.9 mm)后,用离心柱吸附法提取总DNA,构建文库并应用illumina Hiseq 4500做深度(二代、高通量)测序;通过与细菌、真菌、寄生虫和病毒的参考序列做序列对比,分析宏基因组数据,鉴定样本微生物的分类阶元,评估分析具有潜在危害的病原体。结果深度测序共获得clean data 632 GB,排在前3位的病原体为假单胞菌属细菌(0.5611%)、伯克霍尔德菌属细菌(0.4687%)及沙雷氏菌属细菌(4.2420%);可通过输血或血制品传播、具有潜在威胁的病原体则有人细小病毒B19(0.1266%)、利什曼原虫(1.3485%)、刚地弓形虫(0.6158%)等。结论对部分中国无偿献血人群的宏基因组学的分析,可揭示对于我国血液安全造成潜在危害的的病原体,并有针对性地开展新发传染病防控。Objective To analyze the metagenomics and microbiology of voluntary blood donors in China,so as to assess the potential threats of emerging infectious diseases to the safety of blood transfusion.Methods 12300 plasma samples(10 mL each)collected by central blood stations in Chongqing,Liuzhou,Urumqi,Mianyang,Wuhan,Nanjing,Mudanjiang,and Dehong Prefecture area from 2012 to 2018 were subjected to total DNA extraction after ultracentrifugation(32000 rpm/min,centrifugal radius 91.9 mm)in minipools of 160 donations.The metagenomic library was constructed,and deep sequencing was conducted by Illumina Hiseq 4500.By comparing with reference sequences of bacteria,fungi,parasites and viruses,metagenomic data were analyzed,classification of microbes were identified,and potentially harmful pathogens were evaluated.Results A total of 632 GB clean data were obtained by deep sequencing,and the top three pathogens were Pseudomonas(0.5611%),Burkholderia(0.4687%)and Serratia(4.2420%).Pathogens with potential threat which could be transmitted by blood transfusion or blood products were found,such as human parvovirus B19(0.1266%),Leishmania spp(1.3485%)and Toxoplasma gondii(0.6158%).Conclusion Our study analyzed metagenomics of voluntary blood donors in parts of China and revealed pathogens that may cause potential harm to blood safety,which were helpful for targeted prevention and control of emerging infectious diseases.

关 键 词：新发病原体 无偿献血人群 中国 宏基因组学 高通量测序 新发传染病 生物信息学 

分 类 号：R457.1[医药卫生—治疗学] R372[医药卫生—临床医学]