血小板抗原基因多态性与血小板参数的相关性研究  

Correlation between human platelet antigen polymorphisms and platelet parameters

作　　者：周世航[1] 王霓[1] 邵林楠 于卫建[1] 张开立[2] 刘铭[2] 梁晓华[1] ZHOU Shihang;WANG Ni;SHAO Linnan;YU Weijian;ZHANG Kaili;LIU Ming;LIANG Xiaohua(Dalian Blood Center,Dalian 116001,China;Department of Cell Biology,Dalian Medical University)

机构地区：[1]大连市血液中心,辽宁大连116001 [2]大连医科大学细胞生物学教研室

出　　处：《中国输血杂志》2021年第5期461-464,共4页Chinese Journal of Blood Transfusion

摘　　要：目的探讨血小板抗原(human platelet antigens, HPA)基因多态性与血小板参数的关系。方法使用TaqMan-MGB探针实时PCR方法对139名健康中国汉族人进行HPA-2,HPA-3,HPA-5和HPA-15基因分型,同时使用全自动血细胞分析仪检测血小板参数,包括血小板计数(PLT)、血小板体积(MPV)、血小板分布宽度(PDW)以及大型血小板比率(P-LCR)。结果血小板基因型为HPA-2aa的个体的PLT低于HPA-2ab的个体[(234.35±50.10)×10^(3)/μL vs(269.58±41.66)×10^(3)/μL,P<0.05]。血小板基因型为HPA-5aa和HPA-15aa个体的PLT分别高于HPA-5ab和HPA-15ab/bb的个体[HPA-5:(239.36±49.81)×10^(3)/μL vs(200.29±48.02)×10^(3)/μL;HPA-15:(251.00±58.41)×10^(3)/μL vs(231.29±45.20)×10^(3)/μL,P均<0.05]。血小板基因型为HPA-5aa个体的MPV、PDW以及P-LCR低于HPA-5ab的个体[mpv:(10.01±0.72)fL vs(10.94±1.01)fL;PDV:(11.94%±1.35%)vs(14.25%±2.78%);P-LCR:(25.32%±5.03%)vs(31.73%±6.39%),P均<0.05]。HPA-2和HPA-15各基因型的MPV、PDW以及P-LCR的差异无统计学意义(P>0.05)。此外,在HPA-3aa和HPA-3ab/bb各基因型之间的血小板参数差异无统计学意义(P<0.05)。多元线性回归分析表明,HPA-2、HPA-5和HPA-15是血小板计数的独立影响因子,HPA-5是血小板体积的独立影响因子。结论 HPA-2、HPA-5和HPA-15的基因多态性和血小板计数有关,HPA-5基因多态性和血小板体积有关。Objective To investigate the correlation between human platelet antigens(HPA) polymorphisms and platelet parameters.Methods The HPA-2, HPA-3, HPA-5 and HPA-15 genotypes of 139 healthy Chinese Han individuals were detected using TaqMan-MGB probe real-time PCR, while platelet parameters including platelet count(PLT), mean platelet volume(MPV), platelet distribution width(PDW) and platelet-large cell ratio(P-LCR) were measured using hematology cell analyzer. Results The PLT was significantly lower in the individuals with HPA-2aa genotype compared to those with HPA-2ab[(234.35±50.10)×10^(3)/μL vs(269.58±41.66)×10^(3)/μL,P<0.05], while the PLT was significantly higher in individuals with HPA-5aa and HPA-15aa genotypes compared to those with HPA-5ab and HPA-15ab/bb[HPA-5:(239.36±49.81)×10^(3)/μL vs(200.29±48.02)×10^(3)/μL;HPA-15:(251.00±58.41)×10^(3)/μL vs(231.29±45.20)×10^(3)/μL,P<0.05], respectively. The MPV, PDW and P-LCR were significantly lower in individuals with HPA-5aa genotype compared to those with HPA-5ab[mpv:(10.01±0.72)fL vs(10.94±1.01)fL;PDV:(11.94%±1.35%) vs(14.25%±2.78%);P-LCR:(25.32%±5.03%) vs(31.73%±6.39%),P<0.05], but did not differ among the HPA-2 and HPA-15 genotypes. Besides, no significant differences in platelet parameters of individuals with HPA-3aa and HPA-3ab/bb genotypes were notable(P>0.05). HPA-2,-5 and-15 polymorphisms were identified as independent factors for platelet count, and HPA-5 polymorphism was an independent factor for platelet volume, revealed by multiple linear regression analysis.Conclusion HPA-2,-5 and-15 polymorphisms are correlated with platelet count, and HPA-5 polymorphism is correlated with platelet volume.

关 键 词：血小板抗原 血小板参数 单核苷酸多态性 

分 类 号：R457.11[医药卫生—治疗学] R331.143[医药卫生—临床医学]