类孟买血型鉴定及输血策略  

Para-Bombay phenotype identification and transfusion strategy

作　　者：张燕华 陈兰兰 车进 麻静敏 李凤 张嘉洪 ZHANG Yanhua;CHEN Lanlan;CHE Jin;MA Jing-min;LI Feng;ZHANG Jiahong(Beijing Tongzhou Center Blood Station,Beijing 101100,China)

机构地区：[1]北京市通州区中心血站,北京101100

出　　处：《中国输血杂志》2021年第5期541-543,共3页Chinese Journal of Blood Transfusion

基　　金：北京市通州区卫生和计划生育委员会项目(北京市通州区“两高”人才工程专项基金:2017B0400247)。

摘　　要：目的了解类孟买血型血清学及分子生物学特点。方法用血型血清学方法对1例正反定型不符献血者标本检测ABO,H,Lewis血型,抗体筛选、鉴定及交叉配血试验,采用PCR-SSP方法检测ABO血型基因型,采用分子生物学测序进行类孟买血型确认。结果血清学检测结果为Ah分泌型;ABO血型基因检测结果为AO2;FUT1测序显示235G>C,881882delTT,FUT2测序只存在357C>T。结论发现并准确鉴定血型是保证安全输血的必要条件,对于稀有血型献血者应告知并招募其加入稀有血型献血者队伍。Objective To investigate the serological and molecular biological characteristics of para-Bombay phenotype. Methods ABO blood type, H antigen and Lewis blood type in one blood sample with discrepancy between forward and reverse blood typing were detected by serological method. Antibody screening and identification and cross-match test were also performed by serological method. ABO blood group genes were detected by PCR-SSP, and FUT1 and FUT2 genes were sequenced. Results The serological test showed that the Para-Bombay phenotype was Ah secretion. The ABO blood group gene was AO2. FUT1 sequencing revealed two mutations: 235 G>C and 881882 delTT. While FUT2 sequencing showed only one mutation 357 C>T. Conclusion The discovery and accurate identification of blood groups is necessary to ensure the safety of blood transfusion. Blood donors of rare blood groups should be informed and recruited to the team of rare blood donors.

关 键 词：类孟买血型 H抗原 抗-HI FUT基因 

分 类 号：R457.11[医药卫生—治疗学]