Omega-3多不饱和脂肪酸对痤疮模型金黄地鼠皮脂腺增生及PI3K/AKT通路的影响  

Effects of Omega-3 Polyunsaturated Fatty Acids on Sebaceous Gland Hyperplasia and PI3K/Akt Pathway in Acne-induced Golden Hamster

作　　者：吴欣桐 朱明芳[1] 吴淑辉 张曦[1] 张娟娟 WU Xintong;ZHU Mingfang;WU Shuhui;ZHANG Xi;ZHANG Juanjuan(The Second Affiliated Hospital of Hunan University of Chinese Medicine,Changsha,Hunan 410005,China)

机构地区：[1]湖南中医药大学第二附属医院,湖南长沙410005

出　　处：《湖南中医药大学学报》2021年第6期847-851,共5页Journal of Hunan University of Chinese Medicine

基　　金：湖南省临床医疗技术创新引导项目(2020SK51301);湖南省教育厅科学研究项目(18A212);湖南省卫生健康委科研项目(20201862);湖南省研究生科研创新项目(CX20200772);湖南省高层次卫生人才“225”工程培养项目。

摘　　要：目的研究Omega-3多不饱和脂肪酸对痤疮动物模型金黄地鼠皮脂腺斑的影响及相关机制。方法24只金黄地鼠随机分为4组,每组6只:空白组、低剂量鱼油组、高剂量鱼油组、异维A酸阳性对照组,连续灌胃4周,观察金黄地鼠皮脂腺斑大小、组织病理结构、磷脂酰肌醇3-激酶(phosphatidyl inositol 3-kinase,PI3K)以及蛋白激酶B(protein kinase B,AKT)蛋白表达水平。结果干预后,与空白组相比,低剂量鱼油组、高剂量鱼油组、异维A酸阳性对照组皮脂腺斑面积均减小,差异具有统计学意义(P<0.05);低剂量鱼油组、高剂量鱼油组和异维A酸阳性对照组皮脂腺斑组织大小减小、重叠腺叶数减少、排列较空白组疏松;低剂量鱼油组、高剂量鱼油组、异维A酸阳性对照组AKT及PI3K表达水平均比空白组低,差异具有统计学意义(P<0.05)。结论Omega-3多不饱和脂肪酸通过PI3K/AKT通路改善皮脂腺增生、调节皮脂分泌。Objective To study the effect of Omega-3 polyunsaturated fatty acids on the sebaceous spots in golden hamster with acne and its related mechanism.Methods 24 golden hamsters were randomly divided into 4 groups,with 6 in each group:blank group,low-dose fish oil group,high-dose fish oil group,isotretinoin positive control group.The expression levels of PI3 K and Akt,pathological structure and sebaceous plaque size of golden hamsters were observed by continuous intragastry for 4 weeks.Results After intervention,compared with blank group,the area of sebaceous glands in low-dose fish oil group,high-dose fish oil group and isotretinoin positive control group were smaller,the difference was statistically significant(P<0.05);the size of sebaceous plaque tissue in low-dose fish oil group,high-dose fish oil group and isotretinoin positive control group decreased and the number of overlapping gland lobes decreased,and the arrangement was looser than that in blank group.The expression levels of AKT and PI3 K in low-dose fish oil group,high-dose fish oil group and isotretinoin positive control group were lower than those in blank group,and the differences were statistically significant(P<0.05).Conclusion Omega-3 polyunsaturated fatty acids can improve sebaceous gland hyperplasia and regulate sebum secretion through PI3 K/AKT pathway.

关 键 词：痤疮 金黄地鼠 皮脂腺 Omega-3 PI3K AKT 

分 类 号：R275[医药卫生—中医皮科]