毛细管超速离心技术在体外预测大量胎母输血综合征的研究  

Study of Capillary Ultracentrifugation Technique on Prediction of Massive Maternal Transfusion Syndrome in Vitro

作　　者：张源秦 王美茹 徐璐[2] 马怡然[1] 杨融辉 郝一文[1] 程大也[1] 丛桂敏 ZHANG Yuan-qin;WANG Mei-ru;XU Lu(Department of Transfusion,The First Hospital of China Medical University 110000)

机构地区：[1]中国医科大学附属第一医院输血科,110000 [2]沈阳市妇婴医院输血科

出　　处：《临床输血与检验》2021年第3期301-305,共5页Journal of Clinical Transfusion and Laboratory Medicine

基　　金：辽宁省自然科学基金计划重点项目(No.20180530022)资助。

摘　　要：目的探讨毛细管超速离心技术预测大量胎母输血综合征的临床运用,为ABO血型不一致的大量胎母输血提供一种更简便、快速的筛查方法。方法体外模拟胎母输血综合征的血液标本特点,按胎母输血综合征的出血量将O型育龄期非妊娠健康女性体检者EDTA抗凝血分别与A或B型新鲜脐带血按比例混合后室温下孵育1 h,用毛细管超速离心技术分离出远心端胎儿细胞,并进行ABO血型抗原鉴定;采用流式细胞术定量检测胎儿细胞百分比。结果胎母输血综合征出血量达到60 mL和100 mL时,毛细管超速离心技术敏感度、约登指数、阳性预测值和阴性预测值分别为0.8、0.75,0.75、0.7,0.941、0.9375,0.826、0.791。结论毛细管超速离心技术能简单、快速的识别ABO血型不一致的大量胎母输血综合征。Objective To explore the clinical application of capillary ultracentrifugation in predicting massive maternal transfusion syndrome,which is more convenient to screen massive maternal transfusion syndrome with inconsistent blood types.Methods We mixed EDTA-anticoagulant blood of healthy women(type O on childbearing age)with type A/B umbilical cord blood for 1 hour,to simulate maternal transfusion syndrome.Fetal cells were isolated by capillary ultracentrifugation and performed blood group antigens identification.Furthermore,we performed flow cytometry to quantitatively verify the percentage of fetal red blood cells.Results When the fetal bleeding volume reached 60 and 100 mL,the results of sensitivity,Yoden index,positive predictive value and negative predictive value of capillary ultracentrifugation were respectively 0.8,0.75;0.75,0.7;0.941,0.9375 and 0.826,0.791.Conclusions Capillary ultracentrifugation technique can serve as effective method for massive maternal transfusion syndrome with inconsistent ABO blood group.

关 键 词：大量胎母输血综合征 ABO血型不合 毛细管超速离心技术 

分 类 号：R457[医药卫生—治疗学]