五优稻4号水稻香味的遗传分析与SSR分子标记筛选  

Genetic Analysis and SSR Molecular Marker Screening for Fragrance Gene in Rice Wuyoudao 4

作　　者：刘海英[1,2] 杨忠良[2] 刘会[2] 冷春旭[2] 吴立成[2] 徐振华[2] 于艳敏[2] 来永才[3] LIU Hai-ying;YANG Zhong-liang;LIU Hui;LENG Cun-xu;WU Li-cheng;XU Zhen-hua;YU Yan-min;LAI Yong-cai(Postdoctoral Programme,Heilongjiang Academy of Agricultural Sciences,Harbin 150086,China;Institute of Biotechnology,Heilongjiang Academy of Agricultural Sciences,Harbin 150028,China;Heilongjiang Academy of Agricultural Sciences,Harbin 150086,China)

机构地区：[1]黑龙江省农业科学院博士后科研工作站,黑龙江哈尔滨150086 [2]黑龙江省农业科学院生物技术研究所,黑龙江哈尔滨150028 [3]黑龙江省农业科学院,黑龙江哈尔滨150086

出　　处：《黑龙江农业科学》2021年第6期5-9,共5页Heilongjiang Agricultural Sciences

基　　金：黑龙江省自然科学基金面上项目(C2015029);国家重点研发计划(2016YFD0300900);黑龙江省科技厅重点研发项目(GA18B101)。

摘　　要：为促进水稻香味分子标记辅助育种,以优质香稻品种五优稻4号为香味供体亲本,龙粳20为受体亲本进行杂交,根据杂交后代植株香味的表现,对五优稻4号水稻品种香味性状进行遗传分析,并利用SSR分子标记方法筛选与五优稻4号香味连锁的SSR分子标记。结果表明:水稻五优稻4号的香味性状是由单隐性基因控制;通过SSR分子标记引物的筛选,得到了49对能够在两亲本间稳定表现出多态性的引物,用这49对引物筛选由杂交后代构建的选香和无香基因池,得到1个SSR引物RM5647与香味基因紧密连锁,其遗传距离为25.7 cM。In order to promote molecular marker assisted breeding of rice aroma,in this paper,F1 and F2 segreation population derived from a cross between fragrant rice variety Wuyoudao 4 and non-fragrant rice variety Longjing 20 were used to study the inheritance of fragrance trait and initial mapping the fragrance gene by SSR molecular marks in rice.The results showed that the fragrance trait of rice variety Wuyoudao 4 was controlled by one recessive gene and fragrance was recessive and non-fragrance was dominant.Through primer screening,forty-nine pairs of primers showing steady polymorphism in parents were selected.By screening the fragrance and non-fragrance bulked using these primers,one SSR markers RM5647 linked distance of 25.7 cM.

关 键 词：水稻 香味 遗传分析 SSR分子标记 

分 类 号：S51[农业科学—作物学]