山茱萸马钱苷的提取研究  

Study on the Extraction of Loganin from Cornus officinalis

作　　者：张立攀 赵梦瑶 关炳峰 蒋碧伟 王法云 王永 ZHANG Lipan;ZHAO Mengyao;GUAN Bingfeng;JIANG Biwei;WANG Fayun;WANG Yong(Henan Commerce Science Institute Co.,Ltd.,Zhengzhou 450000,China)

机构地区：[1]河南省商业科学研究所有限责任公司,河南郑州450000

出　　处：《河南农业科学》2021年第5期165-172,共8页Journal of Henan Agricultural Sciences

基　　金：河南省科学院重大科研项目聚焦项目(200111002);河南省科学院助推科技成果转化专项(190211014);河南省科学院基本科研业务费项目(200611119)。

摘　　要：为获得超声辅助提取山茱萸中马钱苷的最适工艺参数,以山茱萸为原料,研究了从中提取马钱苷的优化方法。通过超声功率、超声时间、固液比、乙醇含量、试验温度、超声开/关时间和提取循环次数7种单因素试验分析,设计并进行了正交优化试验,结果表明,最佳条件为超声功率200 W、超声时间25 min、固液比1∶30、乙醇含量55%、试验温度40℃、超声开/关时间5 s/5 s、提取循环次数2次,提取结果最好,马钱苷得率为1.513%。验证试验与优化预期结果较为相符。In order to obtain the optimal process parameters of loganin extracted from Cornus officinalis with ultrasonic assistance,Cornus officinalis was used as the raw material to study the optimal method for the extraction of loganin.Based on the results of 7 single-factor experiments,inclucding ultrasonic power,ultrasonic time,material to liquid ratio,ethanol content,test temperature,ultrasonic work/intermittent time,extraction time,orthogonal test of extraction technology of loganin from Cornus officinalis was designed.The optimal conditions were obtained:the ultrasonic power was 200 W,ultrasonic time was 25 min,material to liquid was 1∶30,ethanol content was 55%,test temperature was 40℃,ultrasonic work/intermittent time was 5 s/5 s,and the extraction time was 2.Under the conditions,the loganin yield in the actual verification test could reach 1.513%.The result of proved test was well in line with the expectation of optimization.

关 键 词：山茱萸 马钱苷 提取工艺 超声 单因素试验 正交优化 

分 类 号：S-3[农业科学]