替罗非班通过上调miR-22保护ox-LDL诱导的脐静脉内皮细胞损伤  

Tirofiban protects ox-LDL induced umbilical vein endothelial cells injury through up-regulating miR-22

作　　者：张秋红[1] 刘苗苗 韩天云[1] ZHANG Qiuhong;LIU Miaomiao;HAN Tianyun(Department of Pharmacy,Baoding Second Hospital,Baoding,Hebei 071000,China)

机构地区：[1]保定市第二医院药剂科,河北省保定市071000

出　　处：《中国动脉硬化杂志》2021年第7期573-578,共6页Chinese Journal of Arteriosclerosis

基　　金：保定市科技计划项目(1941ZF007)。

摘　　要：目的探讨替罗非班对氧化型低密度脂蛋白(ox-LDL)诱导的脐静脉内皮细胞(EA.hy926)损伤的影响和可能机制。方法采用低、中、高剂量的替罗非班作用于ox-LDL诱导的EA.hy926细胞,采用细胞计数法(CCK-8)、流式细胞术分别检测细胞活力和凋亡。实时荧光定量PCR(qRT-PCR)检测miR-22表达水平。酶联免疫吸附法(ELISA)检测细胞培养上清液中肿瘤坏死因子α(TNF-α)和白细胞介素6(IL-6)水平。转染miR-22模拟物上调EA.hy926细胞中miR-22表达水平,采用上述方法检测上调miR-22对ox-LDL诱导的EA.hy926细胞损伤的影响。结果替罗非班作用于ox-LDL诱导的EA.hy926细胞后,细胞存活率显著升高,凋亡率显著降低,细胞培养上清液中TNF-α和IL-6含量显著降低,miR-22表达显著升高(P<0.05)。上调miR-22表达后,ox-LDL诱导的EA.hy926细胞培养上清液中TNF-α和IL-6含量显著降低,细胞存活率显著升高,凋亡率显著降低(P<0.05)。结论替罗非班能够减轻ox-LDL诱导的脐静脉内皮细胞凋亡和炎症反应,其机制可能与上调miR-22表达有关。Aim To explore the effect and possible mechanism of tirofiban on oxidized low density lipoprotein(ox-LDL)-induced injury of umbilical vein endothelial cells(EA.hy926).Methods Low,medium and high doses of tirofiban were applied to ox-LDL-induced EA.hy926,and cell viability and apoptosis were detected by cell counting kit(CCK-8)and flow cytometry,respectively.The expression of miR-22 was detected by real-time fluorescent quantitative PCR(qRT-PCR).Enzyme-linked immunosorbent assay(ELISA)was used to detect the levels of tumor necrosis factor alpha(TNF-α)and interleukin-6(IL-6)in cell culture supernatant.miR-22 mimics were transfected into EA.hy926 to up-regulate miR-22 expression,and the effect of up-regulating miR-22 on ox-LDL-induced EA.hy926 cell injury was detected by the above method.Results After tirofiban acted on ox-LDL-induced EA.hy926 cells,the cell survival rate was significantly increased,the apoptosis rate was significantly reduced,and the content of TNF-αand IL-6 in the cell culture supernatant was significantly reduced,the expression of miR-22 was significantly increased(P<0.05).After up-regulating the expression of miR-22,the content of TNF-αand IL-6 in the culture supernatant of EA.hy926 cells induced by ox-LDL was significantly reduced,the cell survival rate was significantly increased,and the apoptosis rate was significantly reduced(P<0.05).Conclusion Tirofiban could alleviate ox-LDL-induced apoptosis and inflammation of umbilical vein endothelial cells,and its mechanism may be related to the up-regulation of miR-22 expression.

关 键 词：替罗非班 脐静脉内皮细胞 miR-22 细胞凋亡 炎症反应 

分 类 号：R5[医药卫生—内科学] R363[医药卫生—临床医学]