烟草幼苗中烟碱转化株的快速筛选  

Rapid screening of nicotine converters from tobacco seedlings

作　　者：李勇[1] 逄涛[1] 陈学军[1] 师君丽[1] 隋学艺 顾华国[1] LI Yong;PANG Tao;CHEN Xuejun;SHI Junli;SUI Xueyi;GU Huaguo(Yunnan Academy of Tobacco Agricultural Sciences,Yuxi 653100,Yunnan,China)

机构地区：[1]云南省烟草农业科学研究院,云南省653100

出　　处：《烟草科技》2021年第6期9-14,共6页Tobacco Science & Technology

基　　金：中国烟草总公司云南省公司科技项目“降低烟叶农残控制技术研究及应用”(2018530000241007)和“基于多维评价的云南烤烟种植区划及可持续发展研究”(2018530000241027)。

摘　　要：为快速鉴别烟草群体中的烟碱转化株,开发了一种基于气相色谱-质谱的烟草幼苗烟碱转化株筛选方法。该方法提供了非孵化和孵化两种策略。采用非孵化策略时,从采样到分析完成仅需5~6 min,该策略可在损失9%非转化株幼苗的前提下完全去除转化株;采用孵化策略时,只需将非孵化策略中所采样品在37℃下封闭培养12 h后进行提取和分析。孵化策略实现了本实验所涉及的幼苗群体(共221株)中转化株和非转化株的完全分离。本研究中所建立的筛选方法方便、高效,可为批量快速识别幼苗群体中烟碱转化株提供技术保障。In order to rapidly identify nicotine converters in tobacco plants,a screening method based on gas chromatography-mass spectrometry was developed,and two strategies(with and without incubation)were proposed.The non-incubation strategy took about 5-6 min from sampling to analysis.With this strategy,the complete removal of converters could be achieved with the sacrifice of 9%non-converters.For the incubation strategy,in-tube samples were cured at 37℃for 12 h before the extraction and analysis.The complete separation of converters from non-converters in 221 seedlings was achieved by using the incubation strategy.The developed method provides a technical support for the fast and efficient identification of nicotine converters from tobacco seedlings.

关 键 词：烟草幼苗 烟碱 降烟碱 转化株 

分 类 号：S572.01[农业科学—烟草工业]