Changes in the Photosynthetic Pigment Contents and Transcription Levels of Phycoerythrin-Related Genes in Three Gracilariopsis lemaneiformis Strains Under Different Light Intensities  

作　　者：CAO Xuexue WANG Haitao ZANG Xiaonan LIU Zhu XU Di JIN Yuming ZHANG Feng WANG Zhendong 

机构地区：[1]Key Laboratory of Marine Genetics and Breeding(Ocean University of China),Ministry of Education,Qingdao 266003,China

出　　处：《Journal of Ocean University of China》2021年第3期661-668,共8页中国海洋大学学报（英文版）

基　　金：This research was supported by the National Natural Sci-ence Foundation of China(No.31872555);the China Agri-culture Research System(No.CARS-50);the Key Pro-gram of Science and Technology Innovation Ningbo(No.2019B10009).

摘　　要：Three Gracilariopsis lemaneiformis strains,including wild type and high-temperature-resistant cultivars 981 and 2007,were studied with the changes in their photosynthetic pigment contents and related gene transcription levels under different light intensities(10,60,100,and 200μmolm^(−2)s^(−1)).The three G.lemaneiformis strains had the following photosynthetic pigments with high-to-low contents:phycoerythrin(PE),phycocyanin(PC),allophycocyanin(APC),and chlorophyll a(Chl a).Among the three strains,cultivar 981 had the highest PE content,followed by cultivar 2007.The PC and APC contents were similar among the three strains,but they were higher in cultivars 981 and 2007 than in the wild type.The Chl a contents in the three G.lemaneiformis strains were equal.A low light intensity(10μmolm^(−2)s^(−1))promoted photosynthetic pigment accumulation in G.lemaneiformis and improved the relative PE gene transcription(peA and peB)in a short period(≤6 d).A high light intensity decreased the PE content.PebA and PebB,which catalyzed phycoerythrobilin synthesis,showed no compensatory upregulation at a low light intensity among the strains except for the wild type.At a high light intensity,transcription levels of pebA and pebB in the three strains were upregulated.This study provided an experimental basis for elucidating the photosynthesis of G.lemaneiformis.As key genes of algal growth,photo-synthesis-related genes served as useful gene markers for screening elite varieties with good traits in breeding.Cultivar 2007 was superior to cultivar 981 in terms of maintaining high pigment levels in a wide range of light intensities,which is the most suitable for aquaculture.

关 键 词：Gracilariopsis lemaneiformis light intensity photosynthetic pigment PE gene transcription 

分 类 号：Q945.11[生物学—植物学]