2011—2019年东台市肠出血性大肠杆菌O157:H7宿主粪便检测结果  

Analysis of feces detection results of EHEC O157:H7 host in Dongtai county from 2011 to 2019

作　　者：金鑫 仲慧[1] 郎睿 朱莹莹 吴银华[1] JIN Xin;ZHONG Hui;LANG Rui;ZHU Ying-ying;WU Yin-hua(Dongtai Municipal Center for Disease Control and Prevention,Jiangsu province,224200,China)

机构地区：[1]东台市疾病预防控制中心,江苏盐城224200

出　　处：《江苏预防医学》2021年第3期287-289,共3页Jiangsu Journal of Preventive Medicine

摘　　要：目的了解东台市动物宿主、腹泻病人粪便中肠出血性大肠杆菌O157:H7检出情况,为制定预防措施提供科学依据。方法2011—2019年,每年5月、7月、9月,采集肠道门诊腹泻病人粪便样本,并于流行前期(5月)和流行后期(9月)采集牛、羊、鸡、猪粪便样本。采用EC肉汤加新生霉素增菌、免疫磁珠富集法浓缩、科马嘉平板分离,经生化反应和血清学鉴定,使用多重引物PCR方法检测毒力因子。结果2011—2019年,共检测宿主粪便样本5244份,检出阳性样本74份,阳性检出率为1.42%。2012年阳性检出率最高,为6.11%(35/573),不同年份检出率差异有统计学意义(χ^(2)=91.21,P<0.05);5月阳性检出率(2.05%)高于9月(1.26%),差异有统计学意义(χ^(2)=4.05,P<0.05);腹泻病人粪便样本中,阳性检出率为0.13%;动物粪便样本中,阳性检出率为2.43%,以牛最高(4.86%),不同类型动物检出率差异有统计学意义(χ^(2)=43.20,P<0.05)。圈养动物阳性检出率(1.21%)低于散养(4.45%),差异有统计学意义(χ^(2)=30.23,P<0.05)。检测菌株74株,stx1、stx2、eaeA、hly基因携带率分别为0、41.89%、97.30%、97.30%,未检出变种基因。结论东台市仍存在肠出血性大肠杆菌流行的潜在危险,应加强动物粪便管理及健康教育干预力度,保护公众健康。Objective To understand the detection rate of EHEC O157:H7 in animal hosts and diarrhea patients in Dongtai area;to provide scientific basis for the formulation of preventive measures.Methods From 2011 to 2019,stool samples of diarrhea outpatients were collected in May,Jul and Sep;while stool samples of cattles,sheeps,chickens and pigs were collected in the pre epidemic period(May)and post epidemic period(Sep).EC meat soup with neovasin was used for culture,Immunomagnetic beads were used for enrichment,Comagaite plate were used for separation;after biochemical reaction and serological identification,the virulence factors were detected by multiplex PCR.Results From 2011 to 2019,a total of 5224 samples were detected,74 were positive,resulting the positive detection rate of 1.41%.The positive detection rate was the highest in 2012(6.11%,35/573),and the detection rates were significant different among different years(χ^(2)=91.21,P<0.05);the positive detection rate in May(2.05%)was higher than that in September(1.26%)(χ^(2)=4.05,P<0.05);for stool samples of diarrhea patients,the positive rate was 0.13%;for animal stool samples,the positive detection rate was 2.43%,the cattle samples had the highest detection rate(4.86%),the detection rates among different types of animals were statistically significant(χ^(2)=43.20,P<0.05).The positive detection rate of captive animals(1.21%)was lower than that of field-raised animals(4.45%)(χ^(2)=30.23,P<0.05).Totally 74 strains were detected,and the positive rates of stx1,stx2,eaeA and hly genes were 0,41.89%,97.30%and 97.30%,respectively;no mutation were detected.Conclusion There is still a potential risk of EHEC epidemic in Dongtai county.The management of animal stools and health education intervention should be strengthened to protect public health.

关 键 词：肠出血性大肠杆菌O157:H7 宿主 粪便 血清学 

分 类 号：R378.2[医药卫生—病原生物学]