氧化亚铁硫杆菌的固定化连续培养试验  

Test Study on Immobilized Continuous Culture of Thiobacillus Ferrooxidans

作　　者：周玥辰 郑志宏 李思云 孙占学[1,2] 王学刚[1] 柯平超 ZHOU Yue-chen;ZHENG Zhi-hong;LI Si-yun;SUN Zhan-xue;WANG Xue-gang;KE Ping-chao(School of Water Resources and Environmental Engineering,East China University of Technology,Nanchang 330013,China;State Key Laboratory of Nuclear Resources and Environment,East China University of Technology,Nanchang 330013,China)

机构地区：[1]东华理工大学水资源与环境工程学院,南昌330013 [2]东华理工大学核资源与环境国家重点实验室,南昌330013

出　　处：《有色金属（冶炼部分）》2021年第6期46-52,共7页Nonferrous Metals（Extractive Metallurgy)

基　　金：国家自然科学基金资助项目(51764001,52064001,41772266);江西省自然科学基金资助项目(20202BABL203022);广东省放射性与三稀资源利用重点实验室开放基金项目(2018B030322009)。

摘　　要：在前人研究基础上,创新性地提出采用50%接种率并逐次降低接种率循环回流的方法,开展氧化亚铁硫杆菌的固定化培养试验。结果表明,该方法可以在接种9次,历时10d内完成细菌固定化,固定化完成时的平均亚铁氧化速率可达0.31~0.61g/(L·h);在同等条件下,固定化细胞对亚铁的平均氧化速率是游离细胞20%接种率时的5倍,40%接种率时的4倍;连续培养阶段,不同材料、不同柱高固定化细菌的亚铁氧化速率最高可达6.67g/(L·h);可选用55cm高沸石柱为后续柱浸试验培养活性菌液。Based on previous studies,aproposal is put forward to carry out immobilization culture experiment of Thiobacillus ferrooxidans by adopting 50%inoculation rate and reducing inoculation rate successively.The test results show that this method can complete immobilization of bacteria within 10 days after inoculation for 9 times,and average oxidation rate of ferrous iron can reach 0.31-0.61 g/(L·h)after complete immobilization.Under the same conditions,average oxidation rate of ferrous iron by immobilized cells is 5 times as high as that of free cells with 20%inoculation rate and 4 times as high as that with 40%inoculation rate.At continuous culture stage,ferrous oxidation rate of highly immobilized bacteria with different materials and different columns can reach 6.67 g/(L·h).The carrier column with height of 55 cm and filled with zeolite which can be selected as subsequent column leaching test to culture active bacteria solution.

关 键 词：氧化亚铁硫杆菌 接种率 固定化 连续培养 亚铁氧化速率 

分 类 号：TL212.12[核科学技术—核燃料循环与材料]