基于肉桂质量标志物(Q-Marker)预测的质量控制研究  

Study on quality control of Cinnamomum cassia based on prediction of Q-Marker

作　　者：陈晓璐 郭振旺 邓家刚 郝二伟[2,3,4] 杜正彩 卢炳达[1] 任鑫 侯小涛[1,2,3] CHEN Xiao-lu;GUO Zhen-wang;DENG Jia-gang;HAO Er-wei;DU Zheng-cai;LU Bing-da;REN Xin;HOU Xiao-tao(School of Pharmacy,Guangxi University of Chinese Medicine,Nanning 530200,China;Guangxi Key Laboratory of Efficacy Study on Chinese Materia Medica,Nanning 530200,China;Guangxi Collaborative Innovation Center of Functional Ingredients of Agricultural Residues,Nanning 530200,China;Guangxi Scientific Experimental Center of Traditional Chinese Medicine,Guangxi University of Chinese Medicine,Nanning 530200,China)

机构地区：[1]广西中医药大学药学院,广西南宁530200 [2]广西中药药效研究重点实验室,广西南宁530200 [3]广西农作物废弃物功能成分研究协同创新中心,广西南宁530200 [4]广西中医药大学广西中医药科学实验中心,广西南宁530200

出　　处：《中草药》2021年第9期2707-2718,共12页Chinese Traditional and Herbal Drugs

基　　金：广西科技基地和人才专项广西中药药效研究重点实验室建设项目(17-259-20);广西科技基地和人才专项(桂科AD19110165);农作物废弃物功能成分研究协同创新中心建设项目(CICAR2017-Y1);广西中医药大学一流学科建设子课题(2019XK103);广西中医药大学·一方制药大学生科技创新课题项目(DXS2019058)。

摘　　要：目的建立一种基于肉桂Cinnamomum cassia质量标志物(quality marker,Q-Marker)预测的质量控制方法,并运用化学计量学综合分析不同产地肉桂质量差异。方法采用高效液相色谱法,通过同时测定肉桂中肉桂醛、香豆素、肉桂醇、肉桂酸及邻甲氧基肉桂醛5种Q-Marker含量,建立肉桂药材的指纹图谱,采用聚类分析、主成分分析等化学计量学手段对27批肉桂药材质量进行评价。结果建立了一测多评法,肉桂醇、肉桂酸及邻甲氧基肉桂醛与内参物肉桂醛的相对校正因子分别为0.1357、0.2115、1.5927;建立了不同产地27批肉桂的HPLC指纹图谱及共有模式,并进行聚类分析、主成分分析和正交偏最小二乘判别分析。结果显示,27批不同产地肉桂可聚为5类,肉桂醛、香豆素是体现3个主产地间样品差异的主要标志性成分,并提示越南产的肉桂成分含量与中国广东、广西产的肉桂存在差异。结论建立的一测多评法能准确、简便地测定肉桂中Q-Marker的含量,不同产地的肉桂药材质量存在一定的差异性。为肉桂质量控制提供了更为科学、全面的依据和基础。Objective To establish a quantitative analysis of Cinnamomum cassia based on the prediction of quality marker(Q-Marker),and to analyze the quality differences of C.cassia from different areas by chemometrics.Methods An HPLC method were developed for the determination of five quality markers including cinnamaldehyde,coumarin,cinnamyl alcohol,cinnamic acid,and o-methoxy cinnamic aldehyde in C.cassia in order to establish the fingerprints of C.cassia.The quality of 27 batches of C.cassia was evaluated by cluster analysis,principal component analysis,and other chemometrics methods.Results The relative correlation factors of cinnamyl alcohol,cinnamic acid,and O-methoxy cinnamic aldehyde were 0.1357,0.2115,and 1.5927.The HPLC fingerprints and common patterns of 27 batches of C.cassia from different origins were established,and cluster analysis,principal component analysis and orthogonal partial least squares discriminant analysis were carried out.The results showed that 27 batches of C.cassia can be clustered into five groups.Cinnamaldehyde and coumarin were the main symbolic components reflecting the difference among the three main producing areas.It was suggested that the content of C.cassia in Vietnam was different from that of C.cassia produced in Guangdong and Guangxi Provinces of China.Conclusion The established QAMS can accurately and easily determine the content of Q-Markers in C.cassia.There are certain differences in the quality of C.cassia from different origins.It provides a more scientific and comprehensive basis for the quality control of C.cassia.

关 键 词：肉桂 质量标志物 一测多评法 化学计量学 香豆素 肉桂醇 肉桂酸 邻甲氧基肉桂醛 肉桂醛 

分 类 号：R284[医药卫生—中药学]