茄子菌核病病原菌鉴定及抗性种质资源筛选  

Pathogen Identification of Eggplant Sclerotinia Rot and Screening of Resistant Germplasm Resources

作　　者：李韦柳[1] 吴永官[1] 甘桂云[1] 蒋雅琴[1] 玉宁 李文嘉[1] 黎炎[1] 康德贤[1] 王益奎[1] LI Weiliu;WU Yongguan;GAN Guiyun;JIANG Yaqin;YU Ning;LI Wenjia;LI Yan;KANG Dexian;WANG Yikui(Institute of Vegetable Research,Guangxi Academy of Agricultural Sciences,Guangxi Key Laboratory of Vegetable Breeding and New Technology Development,Nanning 530007,Guangxi,China)

机构地区：[1]广西农业科学院蔬菜研究所,广西蔬菜育种与新技术研究重点实验室,广西南宁530007

出　　处：《中国蔬菜》2021年第6期85-91,共7页China Vegetables

基　　金：国家自然科学基金项目(31660573);广西科技重大专项(桂科AA17204041);国家现代农业产业技术体系广西大宗蔬菜创新团队项目(nycytxgxcxtd-03-10);广西农业科学院基本科研业务费资助项目(桂农科2018YM13,桂农科2020YM18)。

摘　　要：为明确茄子菌核病病原菌种类及在育种上筛选可利用的菌核病抗性资源,将收集的病原菌进行分离、纯化,结合形态学观察及rDNA-ITS序列分析,并采用田间活体茎接种法对42份茄子种质资源进行菌核病抗性筛选。结果表明:经形态学、分子生物学方法鉴定该病原菌为核盘菌〔Sclerotinia sclerotiorum(Lib.)de Bary〕。不同茄子种质类型抗病性存在明显差异,茄子野生种S.torvum、S.sisymbriifolium、S.anguivi表现免疫(I),4份S.aethiopicum表现为高抗(HR)或抗病(R),4份东南亚初级栽培茄(S.melongena subsp.ovigerum)表现中抗(MR),高级栽培茄均表现感病(S)或者高感(HS),而种间杂种表现免疫(I)。茄子野生近缘种具有菌核病抗性基因,且可通过杂交等手段转育利用。In order to definit the kinds of Sclerotinia rot pathogen and screen out available resources with Sclerotinia rot resistance,this paper separated and purified the collected pathogens,carried out morphology observation and rDNA-ITS sequence analysis,and conducted screen for Sclerotinia rot resistance on 42 eggplant germplasm resources by living stem inoculation method in the fields.The result showed that the pathogen was identified as〔Sclerotinia sclerotiorum(Lib.)de Bary〕by morphology and molecular biology methods.There were obvious differences in disease resistance of different eggplant germplasm types.Three wild eggplants S.torvum,S.sisymbriifolium and S.anguivi were immune(I),4 of S.aethiopicum were resistant(R)or high resistant(HR),and 4 of Southeast Asian primitive cultivars(S.melongena subsp.ovigerum)were moderate resistant(MR),and all of the advanced cultivars were susceptible(S)or high susceptible(HS).All of the interspecific hybrids were immune(I).Wild relative species of eggplant possessed resistant gene to Sclerotinia rot.This gene can be utilized through hybridization,etc.means.

关 键 词：茄子 菌核病 病原菌鉴定 抗性种质筛选 

分 类 号：S436.411[农业科学—农业昆虫与害虫防治]