Zyxin在调节血小板细胞骨架蛋白分布中的作用  

The Role of Zyxin in Regulating Platelet Cytoskeleton Distribution

作　　者：成斌 闫荣 张素勤 杨梦楠 戴克胜 CHENG Bin;YAN Rong;ZHANG Su-Qin;YANG Meng-Nan;DAI Ke-Sheng(Medical College of Soochow University,Jiangsu Institute of Hematology,The First Affiliated Hospital of Soochow University and Collaborative Innovation Center of Hematology,State Key Laboratory of Radiation Medicine and Protection,Key Laboratory of Thrombosis and Hemostasis,Ministry of Health,Suzhou 215006,Jiangsu Province,China)

机构地区：[1]苏州大学医学部,江苏省血液研究所,苏州大学附属第一医院,血液学协同创新中心,放射医学与防护国家重点实验室,卫生部血栓与止血重点实验室,江苏苏州215006

出　　处：《中国实验血液学杂志》2021年第3期876-880,共5页Journal of Experimental Hematology

基　　金：国家自然科学基金(81770117);江苏省科教强卫工程-临床医学中心资助项目(YXZXA2016002)。

摘　　要：目的:探讨斑联蛋白(zyxin)对血小板细胞骨架分布的调节作用。方法:分别取zyxin基因敲除(Zyx^(-/-))和野生型(WT)小鼠的血小板,分离血小板细胞骨架组分,应用Western blot分别检测细胞骨架组分中β-actin、α-actinin、filamin A及myosinⅡA的表达;肌动蛋白聚合诱导剂(Jasplakinolide,Jas)诱导血小板肌动蛋白聚合,同法检测WT和Zyx^(-/-)小鼠血小板细胞骨架组分中相关骨架蛋白的表达;将WT和Zyx^(-/-)小鼠血小板于纤维蛋白原表面进行铺展,用Alexa Fluor 488偶联的鬼笔环肽标记肌动蛋白丝,比较WT和Zyx^(-/-)小鼠血小板荧光强度。结果:Zyxin基因敲除后,血小板主要细胞骨架蛋白β-actin、α-actinin、filamin A及myosinⅡA在静息以及Jas诱导的血小板细胞骨架组分中的含量较WT小鼠血小板明显增多。在纤维蛋白原表面铺展的血小板中,Zyx^(-/-)小鼠血小板肌动蛋白丝含量较WT小鼠血小板增多。结论:Zyxin显著调节血小板细胞骨架分布,在维持血小板细胞骨架稳态中起着重要作用。Objective:To investigate the regulatory effect of zyxin on the distribution of platelet cytoskeleton.Methods:Platelets were isolated from zyxin-knockout(Zyx^(-/-)) and wild type(WT) mice respectively and corresponding platelet cytoskeleton components were separated.The expressions of β-actin,α-actinin,filamin A and myosin II A in cytoskeleton components were detected by Western blot.Actin polymerization was induced by the actin polymerization inducer Jasplakinolide(Jas) in WT and Zyx^(-/-) platelets.Also,the expressions of the cytoskeleton proteins in cytoskeleton components were detected by Western blot.WT and Zyx^(-/-) platelets were allowed to spread on fibrinogen-coated surface.Platelet F-actin was labeled with Alexa Fluor 488-conjugated phalloidin and the fluorescent intensity was compared between WT and Zyx^(-/-) platelets.Results:After zyxin gene was knockout,the expressions of cytoskeleton proteinsβ-actin,α-actinin,filamin A,and myosin II A in resting and Jas-induced platelets were significantly increased.In the platelet spreading on fibrinogen surface,F-actin was increased in Zyx^(-/-) platelets as compared with that in WT platelets.Conclusion:Zyxin significantly regulates the distribution of platelet cytoskeleton,which plays an important role in maintaining platelet cytoskeleton homeostasis.

关 键 词：斑联蛋白 血小板 细胞骨架 小鼠 

分 类 号：R558[医药卫生—血液循环系统疾病]