2型糖尿病患者血浆microRNA差异表达及生物信息学分析  

Differential expression and bioinformatic analysis of microRNA in the plasma of patients with type 2 diabetes mellitus

作　　者：叶莺[1,2] 刘小华[3] 严延生[1,2] Ye Ying;Liu Xiaohua;Yan Yansheng(Fujian Provincial Center for Disease Control,Fuzhou,Fujian 350001,China;Professional Teaching and Training Base,School of Public Health,Fujian Medical University,Fuzhou,Fujian 350001,China;Fuzhou Municipal Center for Disease Control,Fuzhou,Fujian 350004,China)

机构地区：[1]福建省疾病预防控制中心,福州350001 [2]福建医科大学公共卫生学院专业教学实习基地,福州350001 [3]福州市疾病预防控制中心,350004

出　　处：《中华医学遗传学杂志》2021年第6期536-540,共5页Chinese Journal of Medical Genetics

基　　金：福建省科技厅社会发展引导性项目(2017Y0010);福建省科技创新平台建设项目(2019Y2001)。

摘　　要：目的构建2型糖尿病(type 2 diabetes mellitus,T2DM)患者血浆microRNA(miRNA)差异表达谱,探讨miRNA作为T2DM诊疗靶标的可能性。方法采用miRNA芯片技术检测T2DM患者血浆中miRNA差异表达情况,用实时荧光定量PCR(real-time quantitative polymerase chain reaction,RT-qPCR)进行验证,对目标miRNA进行生物信息学分析。结果T2DM患者血浆中122个miRNAs呈差异表达(FC>2,P<0.05),经多源交集筛选得到差异表达miRNAs 14个,其中上调5个,下调9个。RT-qPCR结果显示,血浆中hsa-miR-185-5p及hsa-miR-328-5p在病例组高表达,差异有统计学意义(P<0.05)。生物信息学分析结果提示,这些miRNAs可能通过胰岛素分泌及PI3K-AKT信号通路途径参与T2DM发生发展过程。结论T2DM患者血浆中hsa-miR-185-5p及hsa-miR-328-5p呈差异表达,可能与T2DM发病密切相关。Objective To construct the differential expression profile of microRNA(miRNA)in plasma of patients with type 2 diabetes mellitus(T2DM)and explore the possibility of using miRNA as the target for diagnosis and treatment of T2DM.Methods Agilent miRNA microarray was used to determine the expression profiles of miRNA in the plasma of patients with T2DM(FC>2,P<0.05).The result was verified by real-time quantitative PCR(RT-qPCR).Candidate miRNA was analyzed by bioinformatic tools.Results In total 122 differentially expressed miRNAs were identified.Among these,14 were selected by multi-source intersection screening,which included 5 up-regulated genes and 9 down regulated genes.RT-qPCR showed that the expression of hsa-miR-185-5p and hsa-miR-328-5p have significantly increased in T2DM patients(P<0.05).Bioinformatic analysis suggested that these miRNAs may be involved in the pathogenesis of T2DM through insulin secretion and PI3K-AKT signaling pathway.Conclusion Differential expression of hsa-miR-185-5p and hsa-miR-328-5p in the plasma may be closely associated with the pathogenesis of T2DM.

关 键 词：MICRORNA 2型糖尿病 生物信息学 

分 类 号：R587.1[医药卫生—内分泌] Q811.4[医药卫生—内科学]