基于HPLC指纹图谱和化学模式识别评价不同产地瓜蒌质量  

Quality Evaluation of Trichosanthis Fructus in Different Origin Based on HPLC Fingerprint and Chemical Pattern Recognization

作　　者：王梦娇 唐婷婷 潘林梅[1] 付廷明[1] 李博[1] 朱华旭[1] WANG Mengjiao;TANG Tingting;PAN Linmei;FU Tingming;LI Bo;ZHU Huaxu(Nanjing University of Chinese Medicine,Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization,Jiangsu Research Center of Botanical Medicine Refinement Engineering,Nanjing 210023,China)

机构地区：[1]南京中医药大学,江苏省中药资源产业化过程协同创新中心,江苏省植物药深加工工程研究中心,南京210023

出　　处：《中国现代应用药学》2021年第8期919-924,共6页Chinese Journal of Modern Applied Pharmacy

基　　金：国家自然科学基金项目(81873013)。

摘　　要：目的采用HPLC建立16批瓜蒌药材指纹图谱,结合化学模式识别的方法对不同产地瓜蒌进行区分。方法采集不同产地瓜蒌药材的HPLC指纹图谱,利用“中药色谱指纹图谱相似度评价系统2004 A”进行相似度评价,建立共有模式。以色谱峰信息为数据来源,利用2种化学模式识别方法综合分析不同产地瓜蒌药材的质量。结果建立了不同产地瓜蒌的HPLC指纹图谱共有模式,标定了9个共有峰,相似度基本>0.9。聚类分析和主成分分析能对不同产地药材进行明确区分,表明导致差异的主要化学标记物为香草酸、木犀草苷、腺嘌呤、鸟苷、黄嘌呤5个成分。结论利用聚类和主成分分析明确区分了不同产地瓜蒌,可综合评判不同产地瓜蒌药材的质量。OBJECTIVE To establish an HPLC fingerprint method for 16 batches of Trichosanthis Fructus and distinguish Trichosanthis Fructus from different origins by chemical pattern recognization.METHODS The HPLC fingerprints of Trichosanthis Fructus from different origins were collected.The similarity of the obtained fingerprints were evaluated by the Chinese Medicine Chromatographic Fingerprint Similarity Evaluation System 2004A,then a common pattern was established.Based on its chemical fingerprint information,the quality of Trichosanthis Fructus was comprehensively analyzed by two kinds of chemical pattern recognition methods.RESULTS HPLC fingerprints of Trichosanthis Fructus were established,a total of 9 common peaks were calibrated and the similarity was basically>0.9.Cluster analysis and principal component analysis could clearly distinguish different origins,indicating that the main chemical marker causing the difference was vanillic acid,luteoloside,adenine,guanosine and xanthine.CONCLUSION Cluster analysis and principal component analysis can be used to definitely distinguish different Trichosanthis Fructus and comprehensively evaluate the quality of Trichosanthis Fructus from different origins.

关 键 词：瓜蒌 指纹图谱 相似度评价 聚类分析 主成分分析 

分 类 号：R917.101[医药卫生—药物分析学]