底物类型对厌氧消化过程中胞内外抗生素抗性基因归趋的影响研究  

Effects of substrate types on the fate of intracellular and extracellular antibiotic resistance genes in anaerobic digestion

作　　者：李萍 卢铁东 辛苑[3] 于雯超 魏源送[1,2] 张俊亚[1,2] 申佩弘[3,4] LI Ping;LU Tiedong;XIN Yuan;YU Wenchao;WEI Yuansong;ZHANG Junya;SHEN Peihong(State Key Joint Laboratory of Environmental Simulation and Pollution Control,Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences,Beijing 100085;Department of Water Pollution Control Technology,Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences,Beijing 100085;College of Life Science and Technology,Guangxi University,Nanning 530005;State Key Laboratory of Conservation and Utilization of Subtropical Agricultural Biological Resources,Guangxi University,Nanning 530005)

机构地区：[1]中国科学院生态环境研究中心,环境模拟与污染控制国家重点联合实验室,北京100085 [2]中国科学院生态环境研究中心,水污染控制实验室,北京100085 [3]广西大学,生命科学与技术学院,南宁530005 [4]广西大学,亚热带农业生物资源保护与利用国家重点实验室,南宁530005

出　　处：《环境科学学报》2021年第6期2079-2091,共13页Acta Scientiae Circumstantiae

基　　金：国家重点研发计划项目(No.2018YFD1100603,2019YFC1906502,2016YFD0501405);国家自然科学基金项目(No.A131300051,5180080523)。

摘　　要：厌氧消化(anaerobic digestion,AD)是畜禽粪污和剩余污泥重要的处理处置方式,其在能源回收和消减抗生素抗性基因(antibiotic resistance genes,ARGs)中具有重要的应用前景.底物类型及预处理是影响厌氧消化过程的重要因素,其对ARGs归趋特征的影响尚不明确.本研究针对猪粪、鸡粪和剩余污泥3种典型的环境ARGs源为研究对象,明确不同底物类型对厌氧消化中ARGs归趋的影响特征,结合胞内ARGs(Intracellular ARGs,iARGs)和胞外ARGs(Extracellular ARGs,eARGs)及其微生物群落结构演替的差异,探讨不同底物类型在厌氧消化过程中ARGs的响应特征.结果表明,底物类型对厌氧消化过程中的胞内外ARGs的变化具有显著的影响.高压蒸汽灭菌(120℃,30 min)预处理底物对厌氧消化结束时的总累积甲烷产气量没有显著的提高,鸡粪组中出现产气抑制的现象,这与之前的研究一致.不同底物类型样品中DNA浓度与DNA中16S rRNA基因的丰度之间存在显著相关性(R^(2)=0.663,p<0.05).底物中微生物对厌氧消化中iARGs和eARGs的转归贡献率较小,剩余污泥中的eARG在总ARGs中的比例最高(5.47%).在不同底物类型中iARG和eARGs的变化均显著不同.Anaerobic digestion(AD)is an important treatment method for livestock manure and residual sludge,and has important application prospects in energy recovery and reduction of antibiotic resistance genes(ARGs).Substrate and pretreatment are important factors affecting the anaerobic digestion process,and its influence on the fate of ARGs is unclear.This study was investigated to clarify the effects of substrate on the fate of ARGs in anaerobic digestion focusing on three typical environmental ARGs sources of pig manure,chicken manure and residual sludge,and the response of the fate of ARGs to substrate type is explored considering the intracellular and extracellular ARGs.The results showed that substrate type has a significant impact on the changes of intracellular and extracellular ARGs in anaerobic digestion.The high-pressure steam sterilization(120℃,30 min)pretreated substrate did not significantly increase the total cumulative methane production at the end of anaerobic digestion,and gas production inhibition appeared in the chicken manure group,which was consistent with previous studies.There is a significant correlation between the concentration of DNA and the abundance of 16 S rRNA genes in samples of different substrate types(R^(2)=0.663,p<0.05).The contribution of substrate microorganisms to the fate of iARGs and eARGs in anaerobic digestion is relatively limited,and the proportion of eARG in the residual sludge is the highest(5.47%),while the changes of iARGs and eARGs are significantly different in three types of substrate.

关 键 词：厌氧消化 底物类型 胞内抗性基因 胞外抗性基因 微生物群落 

分 类 号：X705[环境科学与工程—环境工程]