基于多指标正交实验设计分析红芪复方对卵巢切除大鼠骨质疏松的治疗作用  

Analysis of the therapeutic effect of Radix Hedysari compound prescriptions on osteoporosis in ovariectomized rats based on multi-index orthogonal experiment design

作　　者：周湘琳 赵良功[2] 王冬含 施根根 封士兰[1] ZHOU Xiang-lin;ZHAO Liang-gong;WANG Dong-han;SHI Gen-gen;FENG Shi-lan(School of Pharmacy,Lanzhou University,Lanzhou 730000,Gansu Province,China;Department of Orthopedics,The Second Hospital of Lanzhou University,Lanzhou 730000,Gansu Province,China)

机构地区：[1]兰州大学药学院,甘肃兰州730000 [2]兰州大学第二医院骨科,甘肃兰州730000

出　　处：《中国临床药理学杂志》2021年第11期1389-1393,共5页The Chinese Journal of Clinical Pharmacology

基　　金：国家自然科学基金资助项目(81703664);中央高校基本科研业务费专项基金资助项目(lzujbky-2018-57)。

摘　　要：目的探究红芪复方制剂对去势大鼠骨质疏松的治疗作用,并分析最优处方单味药药效贡献地味。方法 (1)利用摘除大鼠双侧卵巢的方法建立绝经后骨质疏松大鼠模型,假手术组仅摘除卵巢周围脂肪组织。将大鼠随机分为6组:处方一组、处方二组、处方三组、模型组、阳性药组和假手术组,每组10只。处方各组按照原生药材10 g·kg^(-1)换算给予中药水提物,阳性药组以戊酸雌二醇(0.2 mg·kg^(-1))给药。假手术组和模型组给予生理盐水。每天给药1次,连续12周。用双能X骨密度仪测定大鼠大鼠全身骨密度(WBBMD)和右股骨密度(RFBD)。(2)将大鼠随机分为11组:处方三组、拆方一组、拆方二组、拆方三组、拆方四组、拆方五组、拆方六组、拆方七组、模型组、阳性药组和假手术组,每组10只。造模和给药方式同上。双能X骨密度仪测定大鼠WBBMD和RFBD,试剂盒法测定大鼠血清碱性磷酸酶(ALP)、抗酒石酸酸性磷酸酶(strACP)、钙(Ca)、磷(P)和白细胞介素-1β(IL-1β),测定子宫指数,三点弯曲实验测定大鼠右股骨最大载荷和弹性模量,对初筛出来的处方进行拆方分析。结果 (1)给药12周后,假手术组、模型组、阳性药组、处方一至三组的大鼠WBBMD分别为(15.10±0.77),(14.44±0.43),(15.32±0.51),(15.24±0.63),(14.91±0.50)和(15.25±0.58)g·dm^(-2);这6组大鼠的RFBD分别为(19.03±2.21),(16.01±1.71),(19.64±0.84),(17.54±1.78),(16.76±1.84)和(17.92±2.58)g·dm^(-2)。处方三组疗效最佳。(2)给药12周后,假手术组、模型组、阳性药组、拆方一至七组、处方三组大鼠血清的ALP分别为(5 536.25±227.21),(4 468.32±49.61),(8 005.93±112.22),(4 948.36±37.49),(5 812.45±230.06),(4 409.11±225.92),(4 174.39±117.06),(7 006.53±319.74),(5 763.25±101.07),(5 616.32±322.54)和(6 287.57±90.29)gropt·mL-1;这11组的strACP分别为(52.21±4.88),(63.02±2.74),(51.75±6.86),(56.70±6.01),(53.66±3.76),(49.03±11.42),(63.67±2.96),(Objective To explore the therapeutic effect of Radix Hedysari compound preparation on osteoporosis in castrated rats, and to analyze the contribution of optimal prescription single drug. Methods(1) The model of postmenopausal osteoporosis rats was established by removing bilateral ovaries. The sham operation group only was removed the adipose tissue around the ovary. Rats were randomly divided into 6 groups: prescription one, prescription two, prescription three group, model group, positive drug group, sham operation group. Ten rats in each group. According to the original medicinal materials 10 g·kg^(-1) conversion of traditional Chinese medicine, herb drugs groups was administrated with water extract, estradiol valerate(0.2 mg·kg^(-1)) was used in the positive group. sham operation and model groups were administered with saline. The whole body bone mineral density(WBBMD)and right femur bone density(RFBD) were measured by dual energy X bone density instrument.(2) Rats were randomly divided into 11 groups: disassembled prescription-1 group, disassembled prescription-2 group, disassembled prescription-3 group, disassembled prescription-4 group, disassembled prescription-5 group, disassembled prescription-6 group, disassembled prescription-7 group, prescription three group, model group, positive drug group, sham operation group. Ten rats in each group. The method of modeling and administration is the same as above. The WBBMD and RFMD were measured by dual energy X bone density instrument. Serum alkaline phosphatase(ALP), tartaric acid phosphatase(strACP), calcium(Ca), phosphorus(P), interleukin-1β(IL-1β), and the uterus index were measured by reagent test kit. The maximum load and elastic modulus of the right femur were measured by three-point bending test. The prescription that have been screened out was disassembled and analyzed. Results(1) After 12 weeks of administration, WBBMD in sham operation group, model group, positive group, prescription one to three groups were(15.10±0.77),(14.44±0.43),(15.32±0.51

关 键 词：红芪复方 骨质疏松 去势大鼠 正交实验 全身骨密度 碱性磷酸酶 抗酒石酸酸性磷酸酶 

分 类 号：R28[医药卫生—中药学]