Porcine Immunoglobulin Fc Fused P30/P54 Protein of African Swine Fever Virus Displaying on Surface of S. cerevisiae Elicit Strong Antibody Production in Swine  

作　　者：Chen Chen Deping Hua Jingxuan Shi Zheng Tan Min Zhu Kun Tan Lilin Zhang Jinhai Huang 

机构地区：[1]School of Life Sciences,Tianjin University,Tianjin 300072,China

出　　处：《Virologica Sinica》2021年第2期207-219,共13页中国病毒学（英文版）

基　　金：supported by the National Key Research and Development Program of China (2018YFD0500500)。

摘　　要：African swine fever virus(ASFV)infects domestic pigs and European wild boars with strong,hemorrhagic and high mortality.The primary cellular targets of ASFV is the porcine macrophages.Up to now,no commercial vaccine or effective treatment available to control the disease.In this study,three recombinant Saccharomyces cerevisiae(S.cerevisiae)strains expressing fused ASFV proteins-porcine Ig heavy chains were constructed and the immunogenicity of the S.cerevisiae-vectored cocktail ASFV feeding vaccine was further evaluated.To be specific,the P30-Fcγand P54-Fcαfusion proteins displaying on surface of S.cerevisiae cells were produced by fusing the Fc fragment of porcine immunoglobulin IgG1 or IgA1 with p30 or p54 gene of ASFV respectively.The recombinant P30-Fcγand P54-Fcαfusion proteins expressed by S.cerevisiae were verified by Western blotting,flow cytometry and immunofluorescence assay.Porcine immunoglobulin Fc fragment fused P30/P54 proteins elicited P30/P54-specific antibody production and induced higher mucosal immunity in swine.The absorption and phagocytosis of recombinant S.cerevisiae strains in IPEC-J2 cells or porcine alveolar macrophage(PAM)cells were significantly enhanced,too.Here,we introduce a kind of cheap and safe oral S.cerevisiae-vectored vaccine,which could activate the specific mucosal immunity for controlling ASFV infection.

关 键 词：African swine fever virus(ASFV) S.cerevisiae Porcine immunoglobulin Fc P30-Fcγ/P54-Fcαfusion proteins 

分 类 号：S852.4[农业科学—基础兽医学]