鱼腥草内生菌Aspergillus sp.GZWMJZ-636次级代谢产物研究  

Secondary metabolites of endophytic fungus Aspergillus sp.GZWMJZ-636 from Houttuynia cordata

作　　者：刘思成 许言超 吴耽 何文文 胡华林 朱伟明[1] 王立平 LIU Si-cheng;XU Yan-chao-;WU Dan;HE Wen-wen;HU Hua-lin;ZHU Wei-ming;WANG Li-ping(State Key Laboratory of Functions and Applications of Medicinal Plants,Guizhou Medical University,Guiyang 550014,China;School of Pharmaceutical Sciences,Guizhou Medical University,Guiyang 550025,China;Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences,Guiyang 550014,China)

机构地区：[1]贵州医科大学省部共建药用植物功效与利用国家重点实验室,贵州贵阳550014 [2]贵州医科大学药学院,贵州贵阳550025 [3]贵州省中国科学院天然产物化学重点实验室,贵州贵阳550014

出　　处：《中草药》2021年第10期2863-2868,共6页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(U1812403);贵州省“百人领军人才”计划;贵州医科大学博士启动基金(博合J字[2020]006号);贵州省科技支撑计划项目([2019]2334)。

摘　　要：目的研究鱼腥草内生真菌Aspergillus sp.GZWMJZ-636的次级代谢产物和抗植物病原菌活性。方法采用硅胶柱色谱、高效液相色谱等方法对化合物进行分离纯化,采用核磁共振谱、质谱和Marfey水解等方法确定化合物的化学结构,采用平板法测试化合物的抗植物病原菌活性。结果从鱼腥草内生真菌Aspergillus sp.GZWMJZ-636的发酵产物中分离鉴定了6个化合物,分别为18-氧代曲普鲁斯太汀B(1)、1,2-裂环-杀锥曲菌素(2)、常现青霉素(3)、短密青霉菌胺F(4)、特来孢菌嗪D(5)、去甲氧基烟曲霉素C(6)。在50μg/mL质量浓度下,化合物2和3对小麦赤霉病菌的抑制率分别为21.4%和20.0%,对辣椒枯萎病菌抑制率分别为18.0%和20.9%。结论化合物1为新的吲哚二酮哌嗪生物碱,鱼腥草内生菌可代谢产生抗植物病原菌活性的化合物。Objective To investigate the secondary metabolites and anti-phytopathogenic activities of the endophytic fungus Aspergillus sp.GZWMJZ-636 from Houttuynia cordata.Methods The secondary metabolites were isolated and purified by column chromatography over the silica gel and HPLC.Their structures were identified by the spectroscopic analysis and Marfey analysis.The anti-phytopathogenic activities were assayed by plate method.Result One new compound,named 18-oxotryprostatin B(1)along with five known compounds,1,2-seco-trypacidin(2),questin(3),brevianamide F(4),terezine D(5),and demethoxyfumitremorgin C(6)were identified.At the concentration of 50μg/mL,compounds 2 and 3 showed anti-phytopathogenic activities against Fusarium graminearum with the inhibition rates 21.4%and 20.0%,and the inhibition rates 18.0%and 20.9%against Fusarium oxysporum,respectively.Conclusion Compound 1 is a new indole diketopiperazine.Endophytes from H.cordata can produce active compounds against plant pathogens.

关 键 词：鱼腥草 曲霉 次级代谢产物 抗植物病原菌活性 吲哚二酮哌嗪 18-氧代曲普鲁斯太汀B 

分 类 号：R284.1[医药卫生—中药学]