基于代谢组学的补阴药药性研究  

Research on property of Yin-tonifying herbs based on metabolomics

作　　者：杨靖 许振鹏 刘艳[1] 康立欣 杨炳友[1] 匡海学[1] YANG Jing;XU Zhen-peng;LIU Yan;KANG Li-xin;YANG Bing-you;KUANG Hai-xue(Key Laboratory of Basis and Application Research of North Medicine,Ministry of Education,Heilongjiang University of Chinese Medicine,Harbin 150040,China)

机构地区：[1]黑龙江中医药大学教育部北药基础与应用研究重点实验室,黑龙江哈尔滨150040

出　　处：《中草药》2021年第10期2987-2995,共9页Chinese Traditional and Herbal Drugs

基　　金：国家重点研发计划项目(2018YFC1707100)。

摘　　要：目的采用代谢组学方法考察补阴药(百合、天冬、麦冬)对正常大鼠能量代谢及相关标志物表达的影响。方法SD大鼠ig百合、天冬、麦冬水煎液,1次/d,连续21 d,收集尿液和肝脏。采用超高效液相色谱与四极杆-静电场轨道阱-线性离子阱三合一组合式质谱仪(UHPLC-Orbitrap-MS)对大鼠尿液样品进行数据采集;基于多元统计分析,建立主成分分析法(principalcomponentanalysis,PCA)和正交偏最小二乘判别分析法(orthogonalpartialleastsquares-discriminantanalysis,OPLS-DA)模型,并采用CompoundDiscoverer3.1软件通过对照品、mz Cloud、人类代谢组学数据库(humanmetabolome database,HMDB)、京都基因与基因组百科全书(Kyoto encyclopedia of genes and genomes,KEGG)等数据库对筛选的代谢物进行鉴定及通路分析;结合通路分析结果选择与物质代谢、能量代谢相关的关键酶进行实验验证。结果与对照组相比,补阴药组大鼠尿液中鉴定出共有差异代谢物26个,表达趋势一致的差异代谢物有16个,其中15个差异代谢物显著下调,补阴药抑制糖代谢、脂肪酸代谢、氨基酸代谢、核苷酸代谢等11条能量代谢相关通路。补阴药能够下调糖代谢、能量代谢相关酶活性,上调脂代谢相关酶活性,从而抑制物质代谢、能量代谢。结论补阴药能够通过下调正常大鼠体内与物质代谢、能量代谢相关代谢物及代谢通路,从而发挥抑制物质代谢、能量代谢的作用。Objective To investigate the effect of yin-tonifying herbs(Lilii Bulbus,Asparagi Radix,and Ophiopogonis Radix)on energy metabolism and expression of metabolites in normal rats.Methods SD rats were ig Lilii Bulbus decoction,Asparagi Radix decoction and Ophiopogonis Radix decoction,once a day for 21 d,urine and liver were collected.Each group data of urine samples was collected by UHPLC-Orbitrap-MS.The high-throughput metabolome data of the urine of rats based on statistical analysis methods such as principal component analysis(PCA)and orthogonal partial least squares-discriminant analysis(OPLS-DA)was obtained to discover the differential metabolites and metabolic pathways through Compound Discoverer 3.1 software with standards and databases such as mz Cloud,human metabolome database(HMDB)and Kyoto encyclopedia of genes and genomes(KEGG).Key enzymes related to material metabolism and energy metabolism were selected for verification based on results of pathway analysis.Results Compared with control group,a total of 26 differential metabolites including 16 metabolites with the same expression trend were identified and 15 metabolites were significantly down-regulated,which inhibited 11 metabolic pathways such as glucose metabolism,fatty acid metabolism,amino acid metabolism,and nucleotide metabolism.Yin-tonifying herbs inhibited material metabolism and energy metabolism through down-regulating expression of enzymes related to glucose metabolism and energy metabolism in liver,and up-regulating expression of enzymes related to lipid metabolism.Conclusion Yin-tonifying herbs show a tendency to inhibit substance metabolism and energy metabolism of normal rats through down-regulating related biomarkers.

关 键 词：补阴药 代谢组学 药性 物质代谢 能量代谢 

分 类 号：R285.1[医药卫生—中药学] R285.5[医药卫生—中医学]