中草药乙醇提取物对5种常见霉菌抑制效果的研究  

Inhibitory effect of Chinese herbal ethanol extract on five kinds of common molds

作　　者：方改霞 耿璐璐 黄金绒 侯凯玉 洪军 李冰冰 FANG Gai-xia;GENG Lu-lu;HUANG Jin-rong;HOU Kai-yu;HONG Jun;LI Bing-bing(School of Life Science&Engineering,Henan University of Urban Construction,Pingdingshan 467036,China;Henan Health Food Engineering&Technology Research Center,Pingdingshan 467036,China)

机构地区：[1]河南城建学院生命科学与工程学院,河南平顶山467036 [2]河南省健康食品工程技术研究中心,河南平顶山467036

出　　处：《河南城建学院学报》2021年第2期84-92,共9页Journal of Henan University of Urban Construction

基　　金：平顶山市科技攻关计划项目(2017007-7.2);河南省高等学校重点科研项目(20A550001)。

摘　　要：探究中草药有效成分对霉菌的抑制效果,以无水乙醇为溶剂,索氏法提取中草药,采用牛津杯法研究中草药提取物对5种霉菌的抑制效果,以0.5%多菌灵和无水乙醇作对照,用SPSS进行数据分析,结合抑菌效果和经济成本,筛选出高效经济的中草药单剂,再进行两种、三种、四种、五种中草药组合配伍,最终筛选出2个效果较佳的中草药(生药)组合复方,即C_(丁香)∶C_(土荆皮)∶C_(花椒)∶C_(黄连)=0.4g/mL∶0.7g/mL∶0.3g/mL∶0.9g/mL,C_(丁香)∶C_(土荆皮)∶C_(花椒)∶C_(甘草)=0.4g/mL∶0.9g/mL∶0.3g/mL∶0.4g/mL。研究结果为开发天然绿色抗霉菌药物提供了科学依据。To explore the inhibitory effect of effective ingredients of Chinese herbal medicine on molds,Anhydrous alcohol was selected as extracting solvent,Soxhlet method is used to extract the active ingredient of Chinese herbal medicine,Oxford cup method used to study the inhibition effect of Chinese herbal medicine effective ingredients on five kinds of contaminated molds,in which 0.5%carbendazim solution and anhydrous ethanol as the control,SPSS was used for data analysis.Based on the results of bacteriostasis,efficacy significance,economic cost and other factors,the suitable Chinese herbal medicine was selected for the combination of two,three,four and five,and finally the two better combination of Chinese herbal medicine was selected out,one combination was clove(0.4g/ml),Golden Larch Bark(0.7g/ml),Pepper(0.3g/ml)and Coptidis Rhizoma(0.9g/ml),the other one was clove(0.4g/ml),Golden Larch Bark(0.9g/ml),Pepper(0.3g/ml)and glycyrrhiza(0.4g/ml).The results of the study will provide the scientific evidences for developing effective green fungicides.

关 键 词：中草药乙醇提取物 污染霉菌 索氏提取法 牛津杯法 抑菌效果 

分 类 号：R285.5[医药卫生—中药学]