陈山红心杉1.5代种子园遗传多样性和子代父本分析  

Genetic diversity and paternity analyses in a 1.5th generation seed orchard of Chenshan red-heart Chinese fir

作　　者：陈兴彬 徐海宁[1] 肖复明[1] 孙世武 娄永峰[1] 邹元熹 徐小强 CHEN Xingbin;XU Haining;XIAO Fuming;SUN Shiwu;LOU Yongfeng;ZOU Yuanxi;XU Xiaoqiang(Jiangxi Provincial Key Lab for Plant Biotechnology,Jiangxi Academy of Forestry,Nanchang 330032,China;Baiyunshan Mountain Forest Farm of Qingyuan District,Ji'an 343000,China;Qingyuan District Forestry Bureau,Ji'an 343000,China)

机构地区：[1]江西省林业科学院,江西省植物生物技术重点实验室,江西南昌330032 [2]吉安市青原区白云山林场,江西吉安343000 [3]吉安市青原区林业局,江西吉安343000

出　　处：《南京林业大学学报（自然科学版）》2021年第3期87-92,共6页Journal of Nanjing Forestry University：Natural Sciences Edition

基　　金：江西省重点研发计划项目(20181ACF60011);江西省林业科技创新项目(201702,201802);江西省林业科学院青年科技人才培养项目(2018521101)。

摘　　要：【目的】陈山红心杉是江西特有的杉木优良种源,其近髓心的木质部为高比例的油亮栗褐色,是工艺建筑和室内装潢极为宝贵的天然材料。对陈山红心杉1.5代种子园进行遗传多样性和子代父本分析,为红心杉种子园的管理提供科学依据。【方法】以江西省青原区白云山林场陈山红心杉1.5代种子园及其子代测定林为研究材料,利用12对SSR引物,对种子园32个亲本及14个无性系的459个子代进行遗传多样性和父本分析。【结果】各引物在亲本群体中检测到等位基因数(Na)为3~7,平均为4.41个;引物在子代群体中检测到的等位基因数(Na)为4~11,平均为6.50个,较亲本群体高2.09个。亲本群体的平均有效等位基因数(Ne)为2.330,子代群体的平均有效等位基因数为2.306。子代群体包含亲本群体所有的等位基因,并检测到25个子代特有的等位基因。子代群体的Shannon’s信息指数(I)=1.004高于亲本群体的0.992,说明子代群体的遗传多样性略高于亲本群体。子代群体和亲本群体的观测杂合度(Ho)分别为0.525和0.571,表明子代群体中杂合单株的比例较亲本有所下降。种子园的多位点异交率(tm)是1.012,单位点异交率(ts)为0.991,说明种子园异交率较高。双亲近交系数(tm-ts)为0.021,表明种子园无性系间近交水平比较低。种子园的有效花粉供体数目(Nep)为7.81。种子园的多位点父本相关性[Rp(m)]和单位点父本相关性[Rp(s)]分别为0.128和-0.016,单位点和多位点父本相关性的差值Rp(s)-Rp(m)=-0.144<0,表明亲本间没有明显的近亲关系。家系间的多位点异交率(tm)变化幅度为0.938~1.200,有10个家系的多位点异交率(tm)大于0。家系间近交系数(tm-ts)变化幅度为-0.127~0.150,9个家系的近交系数大于零,说明这些家系存在近交现象。通过父本分析,在80%的置信水平下确定了325个子代的父本来源,占分析子代总数的70.8%。子代的亲本均不是同【Objective】Chinese fir[Cunninghamia lanceolata(Lamb.)Hook]is an important,fast-growing timber species that is widely distributed in southern China.The Chenshan red-heart Chinese fir is derived from C.lanceolata originating in Jiangxi Province,and its high proportion of shiny,chestnut-brown xylem surrounding the pith is an extremely valuable raw material for craft,architecture and interior decoration.This study aimed to provide a scientific basis for the management of red-heart Chinese fir seed orchards.【Method】We investigated the genetic diversity and analyzed the paternity of a 1.5 th generation seed orchard of red-heart Chinese fir in the Baiyunshan Mountain Forest Farm(Qingyuan District,Jiangxi Province,China).The genetic diversity of 32 parent trees and 459 open-pollinated progenies from 14 clones in a 1.5 th generation clonal seed orchard was analyzed using 12 polymorphic SSR loci.We then conducted a paternity test of the progeny.【Result】We detected 3-7 and 4-11 alleles(Na)at averages of 4.41 and 6.50 per SSR loci,respectively,in the parent and progeny populations.The average number of alleles in the progeny population was 2.09,which was higher than that in the parent population.The effective numbers of alleles(Ne)were 2.330 and 2.306 in the parent and progeny populations,respectively.The progeny population contained all alleles detected in the parent population.In addition,25 alleles detected in the progeny population were undetectable in the parent population.The Shannon information index(I)was 1.004 and 0.992 in the parent and progeny populations,respectively,indicating slightly higher genetic diversity in the progeny than in the parent population.The observed heterozygosity(Ho)was smaller in the progeny,than in the parent population(0.525 vs.0.571),indicating a slightly lower proportion of heterozygosity in the progeny than in the parents.The multilocus(tm)and single-locus(ts)outcrossing rates in the seed orchard were 1.012 and 0.991,respectively,and the inbreeding between parents was not sig

关 键 词：陈山红心杉 种子园 SSR 遗传多样性 父本分析 

分 类 号：S718[农业科学—林学]